To facilitate the storage and distribution of comprehensively annotated, high-quality biospecimens and their derivatives and to provide professional and technical pathology services
Simmons Biomedical Research Building (NB)
NB5.102 - North Campus
6201 Harry Hines Blvd.
Dallas, TX 75235
The Tissue Management Shared Resource (TMSR) serves as a centralized resource that facilitates the storage and distribution of comprehensively annotated, high-quality biospecimens and their derivatives and provides professional and technical histopathological services to Simmons members and collaborators.
The TMSR provides high-quality biospecimen and pathology resources through: preserving and expanding the biorepository and infrastructure with clinical and molecular genomics annotation and integration within a web portal for distribution; development, expanding, and utilizing innovative and routine tissue and cell resources, including tissue microarrays (TMA) and tumor cell lines that will aid in the successful completion of projects by members; and by providing cutting-edge pathology services and expertise in histomorphologic interpretation of human and animal model tumors.
- Tissue Procurement, Banking, and Distribution
Procuring, processing, banking and distribution of human neoplastic and non-neoplastic tissue are performed in the pathology department in Parkland Health & Hospital System and William P. Clements Jr. University Hospital. The TMSR has space in the surgical pathology laboratories where surgical specimens are received and dissected by clinical staff. All tissue is quality-assured via histopathologic examination reviewed by clinical pathologists.
- Clinical-Pathologic Data Collection and Data Integration
Collected specimens are assigned a unique identifier used for annotation and tracking. Each sample can be tracked from procurement, to division into smaller aliquots, to storage, and its distribution to individual investigators. The TMSR Core maintains a secure, password protected, HIPAA compliant, partially web-accessible database recording details of banked specimens. Specimen-related data are captured and stored in a tissue management database, OpenSpecimen, with automatic population from electronic medical records. Members can perform basic sample availability queries of this database in a de-identified manner through a secure web interface to OpenSpecimen.
- Histology, Immunohistochemical, and Tissue Microarray Services
TMSR provides automated immunohistochemical (IHC) staining services using standard, commercially available or investigator-generated antibodies on paraffin-embedded tumor sections. In addition, tissue microarrays are prepared in collaboration with UT Southwestern surgical pathologists. The surgical pathologist who curates each TMA is available for consultation on antibody optimization for IHC procedures and scoring the array.
Technology and Equipment
Leica RM2235 microtome, Boekel Scientific lighted tissue flotation bath, Leica CM-E microscope, Samuka TissueTek tissue processor, Leica EG1160 tissue embedder, Leica Autostainer XL H&E stainer, Isotemp oven, ThermoScientific Slidemate AS slide printer, ThermoScientific Printmate AS150 cassette printer
- Sample Processing
Brady IP300 label printer, six ultra-low freezers (Sanyo, ThermoScientific, and Panasonic) monitored by CDAS (centralized university system), Beckman Coulter Allegra X15R centrifuge, Hettich Zentrifugen Universal 320 R centrifuge
ATA100 tissue arrayer, Boekel slide moat, Dako Autostainer Link 48 IHC and ISH stainer, 4°C refrigerator, -20°C freezer, analytical balance, Millipore water purification system
The use of shared resource specimens and services are available to Simmons Cancer Center members, other UTSW scientists, and external researchers. Fees for histology services and procurement services are subsidized for cancer center members working on oncology projects. Members also have priority for specimens with limited inventory. Please contact Cheryl Lewis, Ph.D., (firstname.lastname@example.org) with any additional questions.