STED (access provided through the Peter O'Donnell Jr. Brain Institute)
Abberior Facility Line STED
Super-resolution · Inverted
Inverted 3D STED super-resolution microscope with adaptive illumination and adaptive optics for imaging deeper specimens. The system can provide approximately 40 nm isotropic resolution in samples up to 70 µm thick, with lateral resolution down to approximately 25 nm.
Manufacturer: Abberior Instruments
Location: ND5.760
Internal rate: $25/hour
$25 autonomous; $65 assisted
Key capabilities: Confocal imaging; 2D STED; 3D STED; Adaptive illumination; Adaptive optics; RESCue; DyMIN; MINFIELD; Automated z-stacks; FRET; Tiling; Live-cell incubation
Access: This system is owned by the Peter O'Donnell Jr. Brain Institute, which provides access to QLMC users.
Technical details
| Imaging modalities | Confocal; 2D STED; 3D STED |
|---|---|
| Super-resolution | STED; approximately 40 nm isotropic resolution; lateral resolution down to approximately 25 nm |
| Specimen types | Live cells; Fixed cells; Tissue; Samples up to approximately 70 µm thick |
| Sample formats | Slides; 35 mm dishes |
| Excitation and depletion | Excitation: 405, 485, 561, and 640 nm; STED depletion: 595 and 775 nm |
| Environmental control | Live-cell imaging: Yes; Temperature: Yes; CO2: Yes; Humidity: Yes; OKO Lab Uno stage-top incubator with premixed gas |
| Special capabilities | Adaptive illumination; Adaptive optics with deformable mirror; RESCue; DyMIN; MINFIELD; Automated z-stacks; FRET; Tiling |
| Objectives | 60x/1.42 oil, 0.15 mm working distance; 60x/1.20 water, 0.28 mm working distance; 60x/1.30 silicon, 0.30 mm working distance |
| Recommended applications | 2D STED; 3D STED; Deep-tissue super-resolution; Organoid imaging; Live-cell super-resolution |