Photo Gallery
Microscopy in action - Explore selected images and videos produced with instruments and analysis resources in the QLMC. Click any image to view @ higher resolution.
Featured images
The butterfly
Large-area imaging
A large region of interest from a Lily flower bud (Carolina microscopy slide #304520) was imaged and stitched on a Leica Stellaris Falcon 8. Objective: HC PL APO CS2 10×/0.40 DRY. Imaged by Marcel Mettlen.
Large-area imaging
A large region of interest from a Lily flower bud (Carolina microscopy slide #304520) was imaged and stitched on a Leica Stellaris Falcon 8. Objective: HC PL APO CS2 10×/0.40 DRY. Imaged by Marcel Mettlen.
Cells expressing H2B-PSmOrange
Photoconversion
Live cells expressing H2B-PSmOrange were used to photoconvert PSmOrange from red to far-red using a binarized image of the Mona Lisa. Although playful rather than scientific, the result highlights the precision of modern microscopes. Acquired on a Nikon spinning-disk confocal with FRAP module using a 100×/1.45 NA objective. Cells were kindly provided by Etai Saponzik, Fiolka Lab.
Photoconversion
Live cells expressing H2B-PSmOrange were used to photoconvert PSmOrange from red to far-red using a binarized image of the Mona Lisa. Although playful rather than scientific, the result highlights the precision of modern microscopes. Acquired on a Nikon spinning-disk confocal with FRAP module using a 100×/1.45 NA objective. Cells were kindly provided by Etai Saponzik, Fiolka Lab.
Live-cell imaging and analysis
Membrane ruffling in RPE-1 cells
Live-cell holotomography
RPE-1 cells were imaged every 6 seconds for 10 minutes on a Tomocube HT-X1 Plus in holotomographic mode. A cropped area from the bottom slice highlights membrane ruffling. Cells were kindly provided by Lizz Maurais, Ly Lab at UT Southwestern.
Live-cell holotomography
RPE-1 cells were imaged every 6 seconds for 10 minutes on a Tomocube HT-X1 Plus in holotomographic mode. A cropped area from the bottom slice highlights membrane ruffling. Cells were kindly provided by Lizz Maurais, Ly Lab at UT Southwestern.
Cellpose-SAM segmentation
Image analysis
U2OS cells were imaged for 48 hours on a Tomocube HT-X1 Plus in holotomographic and brightfield modes, followed by segmentation with Fiji Cellpose-SAM.
Image analysis
U2OS cells were imaged for 48 hours on a Tomocube HT-X1 Plus in holotomographic and brightfield modes, followed by segmentation with Fiji Cellpose-SAM.
Volumetric modeling of a confocal z-stack
3D visualization
An artificial 3D matrix populated with cells was imaged using a Nikon CSU-W1 spinning-disk confocal. The volume was rendered with Imaris 10.2.
3D visualization
An artificial 3D matrix populated with cells was imaged using a Nikon CSU-W1 spinning-disk confocal. The volume was rendered with Imaris 10.2.
Yet another failed cell division
Long-term live imaging
HeLa cells plated in a glass-bottom dish were imaged for 36 hours on the Tomocube HT-X1 Plus. A brightfield sub-stack shows the failed division of a multinucleated HeLa cell. Imaged by Marcel Mettlen.
Long-term live imaging
HeLa cells plated in a glass-bottom dish were imaged for 36 hours on the Tomocube HT-X1 Plus. A brightfield sub-stack shows the failed division of a multinucleated HeLa cell. Imaged by Marcel Mettlen.
Fiji-based image segmentation
Image analysis
Cells were stained for plasma membrane (CDH5/VE-cadherin, magenta), Golgi (red), actin (green), and nuclei (blue). Acquired on a Nikon CSU-W1 spinning-disk confocal using a 10×/0.45 NA objective. Sample preparation and imaging: Steve Spurgin. Image analysis: Marcel Mettlen.
Image analysis
Cells were stained for plasma membrane (CDH5/VE-cadherin, magenta), Golgi (red), actin (green), and nuclei (blue). Acquired on a Nikon CSU-W1 spinning-disk confocal using a 10×/0.45 NA objective. Sample preparation and imaging: Steve Spurgin. Image analysis: Marcel Mettlen.
Large-scale and super-resolution imaging
Frontal mouse brain section – overview
Whole-section overview
A stitched overview showing the scale and organization of the complete frontal mouse brain dataset.
Whole-section overview
A stitched overview showing the scale and organization of the complete frontal mouse brain dataset.
Frontal mouse brain section – detail
Large-scale Airyscan imaging
Imaged on a Zeiss LSM 980 with Airyscan 2 and AI Sample Finder using a Plan-Apochromat 25×/0.8 oil-immersion objective. The 735-tile dataset measures 76,822 × 46,842 pixels, contains approximately 3.6 billion pixels, and was acquired in approximately 40 minutes. Rendered in Imaris 10.2.0. Credit: Chad Smith, Monson Lab.
Large-scale Airyscan imaging
Imaged on a Zeiss LSM 980 with Airyscan 2 and AI Sample Finder using a Plan-Apochromat 25×/0.8 oil-immersion objective. The 735-tile dataset measures 76,822 × 46,842 pixels, contains approximately 3.6 billion pixels, and was acquired in approximately 40 minutes. Rendered in Imaris 10.2.0. Credit: Chad Smith, Monson Lab.
U2OS cells expressing LifeAct
Super-resolution microscopy
Live U2OS cells expressing mNeonGreen-LifeAct to label F-actin. Comparison of TIRF and TIRF-SIM acquired on a GE OMX SR with a 60×/1.42 NA objective. Credit: Runwen Yao.
Super-resolution microscopy
Live U2OS cells expressing mNeonGreen-LifeAct to label F-actin. Comparison of TIRF and TIRF-SIM acquired on a GE OMX SR with a 60×/1.42 NA objective. Credit: Runwen Yao.
Tissue and cellular imaging
Mouse kidney – overview
Tissue imaging
Mouse kidney section stained with WGA-Alexa488, phalloidin-Alexa561, and DAPI. Zeiss LSM 980 Airyscan 2; Plan-Apochromat 20×/0.8 NA; 342 stitched tiles. Credit: Marcel Mettlen.
Tissue imaging
Mouse kidney section stained with WGA-Alexa488, phalloidin-Alexa561, and DAPI. Zeiss LSM 980 Airyscan 2; Plan-Apochromat 20×/0.8 NA; 342 stitched tiles. Credit: Marcel Mettlen.
Murine femur section
Tissue imaging
Femur from a young adult mouse injected with EdU. The frozen section was stained using Click-iT EdU 488 and an anti-aggrecan antibody (CF555). Imaged on a Nikon CSU-W1 spinning-disk confocal with a 20×/0.75 NA objective. Credit: Jingzhu Zhang.
Tissue imaging
Femur from a young adult mouse injected with EdU. The frozen section was stained using Click-iT EdU 488 and an anti-aggrecan antibody (CF555). Imaged on a Nikon CSU-W1 spinning-disk confocal with a 20×/0.75 NA objective. Credit: Jingzhu Zhang.
Mouse nodose ganglion
Multiplex RNAscope
Fixed mouse nodose ganglion labeled with multiplex RNAscope for Phox2b (Opal 570), Cck1r (Opal 520), and DAPI. Zeiss LSM 880 Airyscan; 63×/1.4 NA. PMID: 34605820. Credit: Laurent Gautron.
Multiplex RNAscope
Fixed mouse nodose ganglion labeled with multiplex RNAscope for Phox2b (Opal 570), Cck1r (Opal 520), and DAPI. Zeiss LSM 880 Airyscan; 63×/1.4 NA. PMID: 34605820. Credit: Laurent Gautron.
Colon section
Tissue imaging
Mouse Colon was stained for mitochondria (TOM-20; red) and nuclei (DAPI; blue). A large region of interest was imaged and stitched on a Leica Stellaris Falcon 8. Objective: HC PL APO CS2 63x/1.40 oil. Sample prep: Shaika Shanbagh, Galia lab; Imaged by Marcel Mettlen.
Tissue imaging
Mouse Colon was stained for mitochondria (TOM-20; red) and nuclei (DAPI; blue). A large region of interest was imaged and stitched on a Leica Stellaris Falcon 8. Objective: HC PL APO CS2 63x/1.40 oil. Sample prep: Shaika Shanbagh, Galia lab; Imaged by Marcel Mettlen.