News
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- 11/2020: New spinning disk confocal
The Live Cell Image Facility is updating its microscope suite: the well-used Andor spinning disk confocal has been replaced with a new Nikon CSU-W1 SoRa spinning disk confocal. The microscope is located in NL5.120R.
supported by NIH 1S10OD028630-01
- 09/2021: New LCIF Director
After 17 years as director of the LCIF, Dr. Kate Luby-Phelps has decided to reduce her workload by turning the facility over to Dr. Marcel Mettlen (mail:marcel.mettlen@utsouthwestern.edu). Dr. Mettlen has more than fifteen years of light microscopy experience and has been learning the ways of the LCIF since January 2020. Dr. Luby-Phelps will continue as director of the Electron Microscopy Core Facility and will remain associated with the LCIF as a staff member.
Unaffected by this change in leadership, the LCIF will continue to offer affordable access to a variety of state-of-the-art optical microscopes. Imaging modalities include laser-scanning and spinning-disk confocal, multiphoton, wide-field deconvolution, TIRF, and more. In addition, the LCIF offers help and advice with sample preparation, image quantification, automation of data analysis and basic microscope maintenance.
New widefield epi-fluorescence microscope
The Live Cell Image Facility is adding a new microscope to its suite: inverted Nikon Eclipse Ti widefield epi-fluorescence microscope.
- 12/2021: LCIF becomes the Quantitative Light Microscopy Core
To better encompass the use of our microscopes and services we provide, the core facility has adopted a new name: Quantitative Light Microscopy Core (QLMC). Although the name changes, our mission remains the same!
- 01/2022: Graduate course and our photo gallery went live
2022 graduate course "Multiscale Microscopy for Biomedical Research"
Feel free to follow our "multiscale microscopy for biomedical research" graduate course!
Dates and time: 01/10 - 05/06; Mondays and Fridays from 1:30 to 3pm.
Place: Contact Kate Phelps (kate.phelps@utsouthwestern.edu) to obtain the zoom link
Hands-on labs and demos are limited to registered graduate students. Auditors will be accommodated if space allows. Contact Kate Phelps (kate.phelps@utsouthwestern.edu) to sign up for the waiting list.
Call for images to be featured on our photo gallery
The QLMC would like to highlight your research and is looking for images to be featured in our website's photo gallery. If you have data that was acquired on one of our microscopes and you’d like to contribute to this gallery, please submit your image(s) with a quick description (microscope, magnification, sample type, fluorescent probes, …. ) to Marcel Mettlen (Email). We are happy to link this image to your lab's website or publication (if applicable) to create some buzz. Looking forward seeing your beautiful/colorful/amazing/funny images! Thank you.
- 02/2022: Demo the Olympus FV3000 laser scanning microscope
Demo the Olympus FV3000 laser scanning microscope!
Virtual informational workshop: 02/24 at 12:00pm
Please contact Marcel Mettlen (Marcel.Mettlen@utsouthwestern.edu) for zoom link.
Demo Dates: 03/03 - 03/10
Place: NL5.120RFor more information see: flyer (PDF) or visit the Olympus webiste: FV3000
- 03/2022: Demo of the Zeiss LSM980 with AiryScan2
Demo the Zeiss LSM980 with Airyscan2!
Webinar: 03/11 at 12:00pm
For more information and registratation, please visit: Zeiss LSM980
Demo Dates: 03/21 - 03/30
Place: NL5.120R
- 05/2022: Fiji workshop; Livecyte (short poll)
Announcing the 2022 ImageJ/Fiji Workshop
Dates: May 31, June 7, June 14
Time: 9:00am to noon
Room: D1.502
Instructors: Marcel Mettlen (Director, QLMC) and Kate Luby-Phelps (Director, EMCF)May 31 - A Basic Introduction to Digital Imaging & Image Analysis with ImageJ/Fiji
June 7 - Using ImageJ/Fiji for Specific Applications
June 14 - An Introduction to Scripting/Macro WritingThis workshop is open to all investigators at UT Southwestern. There is no need to RSVP, just show up. Please bring your laptop and have Fiji installed before you come. Links to the downloads with installation instructions are here: https://imagej.net/Fiji/Downloads
Possibly adding new capabilites to the QLMC
The Livecyte is a microscope system able to capture a large amount of high-content images of even the most sensitive cells. With its integrated AI software, the Livecyte monitors and characterizes cell proliferation, cell lineages, motility, morphology and more, from 96 wells to single cells. Currently, no device on campus compares to this system in terms of data quality and amount of information extracted. Below are just 2 examples:
To gage interest, please complete the follwing short poll: Livecyte
Thank you!
- 12/2022: Demo of the Andor Dragonfly spinning-disk confocal and Imaris workshop
Demo of the Andor Dragonfly spinning-disk confocal and Imaris workshop
We are excited to demo Andor's flagship spinning-disk confocal microscope this January: the DragonFly 600. The Dragonfly is an advanced multi-modal imaging system, capable of fast and gentle confocal, widefield, TIRF, and nano-scale super-resolution microscopy.
Demo Dates: 01/09 - 01/13
Place: TBD
Webinar: 01/09 at 12:00pm in NL6.125. Light refreshments will be provided.
For more information see: flyer (PDF) or visit Andor webiste: DragonFly600
If you have questions, feel free to contacte Marcel Mettlen (marcel.mettlen@utsouthwestern.edu) or Ryan Robinson (Email).
- 01/2023: 2023 Graduate course "Multiscale Microscopy for Biomedical Research”
2023 graduate course "Multiscale Microscopy for Biomedical Research"
Feel free to follow our "multiscale microscopy for biomedical research" graduate course!
Dates and time: 01/11 - 05/06; Wednesdays and Fridays from 1:30 to 3pm.
Place: Contact Kate Phelps (kate.phelps@utsouthwestern.edu) for more information.
Hands-on labs and demos are limited to registered graduate students. Auditors will be accommodated if space allows. Contact Kate Phelps (kate.phelps@utsouthwestern.edu) to sign up for the waiting list.
- 08/2023: New widefield microscope
The Quantitative Light microscopy core is updating its microscope suite by adding a widefield microscope for epifluorescence and histological stains (color camera). The system is fully motorized for xyz scans. Furthermore, the full enclosure incubator and perfect focus system allows for long-term acquisition of live cells. The microscope is located in NL5.120R.
- 12/2023: New, dual-camera spinning disk confocal
The Quantitative Light Microscopy Core is updating its microscope suite: The heavily used Nikon CSU-W1 SoRa spinning disk confocal finally gets some relief: a new Nikon CSU-W1 spinning disk confocal with 2 cameras. Closely matching the configuration of the “old” system, the new spinning disk does not have the FRAP/SoRa modules, but comes with an additional 594nm laser line and 2 cameras for true simultaneous channel acquisition. The microscope is located in NL5.120R.
- 01/2024: New, fully loaded Zeiss LSM980
The Quantitative Light Microscopy Core is adding another high-end system to its microscope suite: a fully specced LSM980. This state-of-the-art setup will meet all your fluorescent imaging needs. Equipped with 8 laser lines (405, 445, 488, 514, 561, 594, 639 and 730nm), it allows for multi-color imaging. Combined with the 32-channel spectral detector, the system can easily handle your multiplexed samples. In addition, the system comes with Zeiss’ proprietary AI sample finder, greatly facilitating and accelerating the acquisition of large tissue sections. The Airyscan2 module allows for fast laser scanning confocal imaging which is supported by a high-speed piezo z-stage. Alternatively, the Airyscan2 module allows for easy-to-implement super-resolution data acquisition, effectively doubling the optical resolution (down to 120nm). The microscope is located in NL5.120LA.