Lipid and Metabolite Mass Spectrometry Facility

The Center for Human Nutrition lipid mass spectrometry lab is in the Green Science Building (L4.308) and occupies 2,000 square feet of newly renovated, state-of-the-art laboratory space housing over 16 mass spectrometers. This is a newly constructed open-concept lab with a modular design that allows equipment to relocated with minimal effort or disruption based on current needs.

Other than two chemical fume hoods and a sink, there are no fixed structures in the lab. Nitrogen and ultra-pure air are delivered from an external 6,000-gallon liquid nitrogen dewer and twin (redundant) Atlas Copco SF22+ scroll compressors with CD35+ dryers. All gasses are delivered through a fully stainless steel, medical grade piping network.

The lab is temperature controlled through a combination of house HVAC and four in-room water chilled CRAC units. Mechanical services (gas, electrical, and vent snorkels) are delivered primarily through ceiling mounted quick connect gas fittings, twist-lock power cords, and snorkels with additional electrical service along the walls.

There is currently 64 feet of mobile benches (AT Villa Ergo) for sample preparation, instrument maintenance, and other activities related to lipid mass spectrometry.

SCIEX 4000 QTRAP Triple Quadrupole Mass Spectrometer  
Two SCIEX API 5000 Triple Quadrupole Mass Spectrometers  
SCIEX TripleTOF© 6600 Quadrupole time-of-flight Mass Spectrometer  
SCIEX QTRAP© 6500 Triple Quadrupole/Ion Trap Mass Spectrometer  
SCIEX QTRAP© 4500 Triple Quadrupole/Ion Trap Mass Spectrometer 
Agilent 7890A/5975c  
Agilent 7890C/5975c 
Agilent 6890N/5973  
Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ 1M Mass Spectrometer 
Thermo Scientific™ Q Exactive™ GC Orbitrap™ Mass Spectrometer 
SCIEX 3200 QTRAP Triple Quadrupole Mass Spectrometer 
Agilent 6980 Gas Chromatograph 
Thermo Ultimate 3000 UHPLC 

Sample Preparation:

  • Hamilton Starlet liquid handling robot
  • Biotage 96-well positive pressure SPE manifold
  • Biotage 96-well nitrogen dryer
  • (3) Thermo-Pierce 27-position nitrogen dryers
  • (2) Labconco 50-position RapidVap Vertex nitrogen dryers
  • Omni BeadRuptor bead beater/homogenizer
  • (2) Benchtop centrifuges
  • (2) Micro centrifuges

Sample Storage:

  • (2) -80 °C freezers
  • (2) -20 °C solvent-safe freezers
  • Lab refrigerator

Air and Nitrogen Supply:

  • Parker LCMS 5000 ultra-pure air and nitrogen generator to supply the API 5000 instruments
  • 6000-gallon liquid nitrogen dewar to supply nitrogen to the L4.308 CHN mass spectrometry laboratory.
  • (2) Atlas Copco SF22+ scroll compressors each with CD35+ air dryers and particle filters to generate “zero” air to L4.308 CHN mass spectrometry laboratory.