Resources
Quick Start Guide to PPMS
The QLMC uses PPMS to manage instrument reservations, training requests, and billing. New users must register for a QLMC account through PPMS:
- View registration instructions for UT Southwestern users.
- View registration instructions for external users.
- If you need assistance creating your account, please contact sabia.sabharwal@utsouthwestern.edu.
Training Videos
Before using the Zeiss LSM 880, please watch the following training videos produced by Jason Kirk, director of the Optical Imaging and Vital Microscopy Core:
- Introduction to Confocal Microscopy
- Training: Carl Zeiss LSM 880 Confocal Microscope (begin watching at 2:20)
- Training: Airyscan and Airyscan FAST
Reference Library
- Microscopy Basics
- Imaging Best Practices and Common Pitfalls
- The Good, the Bad and the Ugly (Nature)
- What’s in a Picture? The Temptation of Image Manipulation (Journal of Cell Biology)
- Seeing Is Believing? A Beginners’ Guide to Practical Pitfalls in Image Acquisition (Journal of Cell Biology)
- Tutorial: Guidance for Quantitative Confocal Microscopy (Nature Protocols)
- Fluorescent Proteins
- Quantum Dots
- Potentials and Pitfalls of Fluorescent Quantum Dots for Biological Imaging (Trends in Cell Biology)
- Advances in Fluorescence Imaging with Quantum Dot Bio-Probes (Biomaterials)
- Colocalization of Fluorophores
- A Practical Guide to Evaluating Colocalization in Biological Microscopy (American Journal of Physiology–Cell Physiology)
- A Guided Tour into Subcellular Colocalization Analysis in Light Microscopy (Journal of Microscopy)
- Quantitative Fluorescence Co-localization to Study Protein–Receptor Complexes (Methods in Molecular Biology)
- FRET (Förster Resonance Energy Transfer)
- Fanciful FRET (Science’s STKE)
- Cerulean, Venus, and VenusY67C FRET Reference Standards (Biophysical Journal)
- Investigating Protein–Protein Interactions in Living Cells Using Fluorescence Lifetime Imaging Microscopy (Nature Protocols)
- TIRF (Total Internal Reflection Fluorescence Microscopy)
- Total Internal Reflection Fluorescence Microscopy: Technical Innovations and Novel Applications (Current Opinion in Biotechnology)
- Overcoming Optical Challenges to Live-Cell TIRF Microscopy (Photonics Media)
- Super-Resolution Imaging
- A New Wave of Cellular Imaging (Annual Review of Cell and Developmental Biology)
- Navigating Challenges in the Application of Superresolution Microscopy (Journal of Cell Biology)
- Strategic and Practical Guidelines for Successful Structured Illumination Microscopy (Nature Protocols)