Resources

Quick Start Guide to PPMS

The QLMC uses PPMS management software for all booking, training, and billing purposes. New users must register for an account with the QLMC on PPMS:

• If you work at UTSW, CLICK HERE for instructions.
  For all others, CLICK HERE.
• For all issues related to your account creation, don't hesitate to contact Sabia Sabharwal directly.

Training Videos

Please watch these excellent training videos for the Zeiss LSM880, produced by Jason Kirk, director of the OiVM:

• Introduction to confocal microscopy 
• Basic operations of the LSM880 (start watching at 2:20min)
• Operating the AiryScan on the LSM880

Reference Library

• Microscopy Basics
  • Microscopy Primer
• Imaging Do's and Don'ts
  • The good, the bad and the ugly (Nature)
  • What's in a picture? The temptation of image manipulation (JCB)
  • Seeing is believing? A beginner's guide to practical pitfalls in image acquisition (JCB)
  • Tutorial: guidance for quantitative confocal microscopy (Nature Protocols)
• Fluorescent Proteins
  • Fluorescent protein spectrum viewer
• Quantum Dots
  • Potentials and pitfalls of fluorescent quantum dots for biological imaging (Trends in Cell Biology)
  • Advances in fluorescence imaging with quantum dot probes (Science)
• Colocalization of Fluorophores
  • A practical guide to evaluating colocalization in biological microscopy (Science and Engineering Ethics)
  • A guided tour into subcellular colocalization analysis in light microscopy (Wiley Online Library)
  • Quantitative Fluorescence Co-localization to Study Protein–Receptor Complexes (Methods in Molecular Biology)
• FRET (Förster resonance energy transfer)
  • Fanciful FRET (Science Signalling)
  • Cerulean, Venus, and VenusY67C FRET Reference Standards (Biophysical Journal)
  • Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy (Nature Protocols)
• TIRF (Total internal reflection fluorescence microscopy)
  • Total internal reflection fluorescence microscopy: technical innovations and novel applications (Current Opinion in Biotechnology)
  • Overcoming Optical Challenges to Live-Cell TIRF Microscopy (Photonics Media)
• Super-Resolution Imaging
  • A New Wave of Cellular Imaging (Annual Review of Cell and Developmental Biology)
  • Navigating challenges in the application of superresolution microscopy (Journal of Cell Biology)
  • Structured Illumination (Nature Protocols)