Publications

2021

Tso, S.C. & Brautigam, C.A. (2021). Measuring the KD of protein-protein interactions using microscale thermophoresis. Methods Mol. Bio., 2263, 161-181.

2020

Brautigam, C.A., Tso, S.C., Deka, R.K., Liu, W.Z., & Norgard, N. (2020). Using modern approaches to sedimentation velocity to detect conformational changes in proteins. Eur. Biophys. J., 49, 729-743.

2019

Tso, S.C., Brautigam, C.A., Chaturvedi, S.K., Bollard, M.T., Krynitsky, J., Kakareka, J.W., Pohida, T.J., Zhao, H., & Schuck, P. (2019). Enhanced sample handling for analytical ultracentrifugation with 3D-printed centerpieces. Anal. Chem., 91, 5866-5873.

2018

Wands, A.M., Cervin, J., Huang, H., Zhang, Y., Youn, G., Brautigam, C.A., Matson, Dzebo M., Björklund, P., Wallenius, V., Bright, D.K., Bennett, C.S., Wittung-Stafshede, P., Sampson, N.S., Yrlid, U., Kohler, J.J. (2018). Fucosylated molecules competitively interfere with cholera toxin binding to host cells. ACS Infect. Dis., 4, 758-770.

Tso, S.-C., Chen, Q., Vishnivetskiy, S.A., Gurevich, V.V., Iverson, T.M. & Brautigam, C.A. (2018). Using two-site models to analyze microscale thermophoresis data. Anal. Biochem., 540-541, 64-75.

2016

Brautigam, C.A., Deka, R.K., Liu, W.Z., & Norgard, M.V. (2016). The Tp0684 (MglB-2) lipoprotein of Treponema pallidum: a glucose-binding protein with divergent topology. PLoS One, 11, e0161022.

Scheuermann, T.H., Padrick, S.B., Gardner, K.H., & Brautigam, C.A. (2016). On the acquisition and analysis of microscale thermophoresis data. Anal. Biochem., 496, 79-93.

2015

Brautigam, C.A. (2015). Fitting two- and three-site binding models to isothermal titration calorimetric data. Methods, 76, 124-136.

Scheuermann, T.H. & Brautigam, C.A. (2015). High-precision, automated integration of multiple isothermal titration calorimetric thermograms: new features of NITPIC. Methods, 76, 87-98.

2014

Ayaz, P., Munyoki, S., Geyer, E.A., Piedra, F.A., Vu, E.S., Bromber, R., Otwinowski, Z., Grishin, N.V., Brautigam, C.A., & Rice, L.M. (2014). A tethered delivery mechanism explains the catalytic action of a microtubule polymerase. eLife, 3, e03069.

Ghirlando, R., Zhao, H., Balbo, A., Piszczek, G., Curth, U., Brautigam, C.A., & Schuck, P. (2014). Measurement of the temperature of the resting rotor in analytical ultracentrifugation. Anal. Biochem., 458, 37-39.

Ouyang, Z., Zhou, J., Brautigam, C.A., Deka, R., & Norgard, M.V. (2014). Identification of a core sequence for the binding of BosR to the rpoS promoter region in Borrelia burgdorferi. Microbiology, 160, 851-862.

Zhang, X., Wu, J., Du, F., Xu, H., Sun, L., Chen, Z., Brautigam, C.A., Zhang, X., & Chen, Z.J. (2014). The cytosolic DNA sensor cGAS forms an oligomeric complex with DNA and undergoes switch-like conformational changes in the activation loop. Cell Rep., 6, 421-430.

2013

Ni, L., Li, S., Yu, J., Min, J., Brautigam, C.A., Tomchick, D.R., Pan, D., & Luo, X. (2013). Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5. Structure, 21, 1757-1768.

Wang, Y., Pascoe, H.G., Brautigam, C.A., He, H., & Zhang, X. (2013). Structural basis for activation and non-canonical catalysis of the Rap GTPase activating protein domain of plexin. eLife, 2, e01279.

Ghirlando, R., Balbo, A., Piszczek, G., Brown, P.H., Lewis, M.S., Brautigam, C.A., Schuck, P., & Zhao, H. (2013). Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references. Anal. Biochem., 440, 81-95.

Brautigam, C.A., Padrick, S.B., & Schuck, P. (2013). Multi-signal sedimentation velocity analysis with mass conservation for determining the stoichiometry of protein complexes. PLoS One, 8, e62694.

Zhou, H., Ghirlando, R., Piszczek, G., Curth, U., Brautigam, C.A. & Schuck, P. (2013). Recorded scan times can limit the accuracy of sedimentation coefficients in analytical ultracentrifugation. Anal. Biochem., 437, 104-108.

2012

Keller, S., Vargas, C., Zhao, H., Piszczek, G., Brautigam, C.A., Schuck, P. (2012). High-precision isothermal titration calorimetry with automated peak-shape analysis. Anal. Chem., 84, 5066-5073.

Ayaz, P., Ye, X., Huddleston, P., Brautigam, C.A., Rice, L.M. (2012). A TOG:αβ-tubulin complex structure reveals conformation-based mechanisms for a microtubule polymerase. Science, 337, 857-860.

2011

Padrick, S.B. & Brautigam, C.A. (2011). Evaluating the stoichiometry of macromolecular complexes using multisignal sedimentation velocity. Methods, 54, 39-55.

Brautigam, C.A. (2011). Using Lamm-Equation modeling of sedimentation velocity data to determine the kinetic and thermodynamic properties of macromolecular interactions. Methods, 54, 4-15.