Beckman-Coulter Analytical Ultracentrifuge
The AUC is used to apply a strong centrifugal field to solutions of macromolecules. It is equipped with both absorption and interference optics for the detection of the resulting concentration gradients.
Determination of molar mass, oligomerization state, stoichiometry, association constant.
This consists of a light-scattering detector and a differential refractive index detector. Laser (or LED) light illuminates the sample as it passes through flow cells. Can be used with DLS.
Determination of molar mass, hydrodynamic radius, and extinction coefficients.
J-815 Circular Dichroism Spectrometer
The CD Spectrometer illuminates the sample with UV and/or visual radiation and measures the differential absorption of the right- and left-circularly polarized light.
Estimation of secondary structure content, determinations of protein stability and of association constant of ligands exhibiting CD.
NanoTemper Microscale Thermophoresis
The MST directs an infrared laser onto a capillary that is filled with a fluorescently labeled molecule and an unlabeled binding partner. Changes in how the labeled molecule moves into or out of the heated volume provide insight into the interaction.
Determination of association constants and other thermo-dynamic information.