BD LSR II
A high-performance conventional flow cytometer capable of advanced multicolor analysis using multiple lasers and optimized detector configurations.
Best suited for:
- Complex multicolor panels
- High-sensitivity detection of rare populations
- Advanced immunophenotyping
Access: Available for trained users (24/7 self-operated)
Technical Highlights:
- Multiple laser configurations (355 nm UV, 405 nm violet, 488 nm blue, 640 nm red)
- Expanded detector capacity for high-parameter experiments
- High sensitivity for low-abundance markers
Technical Specifications
BD LSR II (4-Laser: 405 nm, 488 nm, 532 nm, 633 nm)
Channel and Dye Compatibility Reference
This reference document is configured for a BD LSR II system equipped with:
- 405 nm (Violet)
- 488 nm (Blue)
- 532 nm (Green – PE optimized)
- 633 nm (Red)
Exact detector filters may vary by instrument. Always confirm configuration with the Flow Core.
| Laser | Detector Filter | Compatible Fluorochromes (BD + Other Vendors) |
|---|---|---|
| 405 nm (Violet) | 450/50 | BV421, Pacific Blue, eFluor 450, VioBlue, Super Bright 436 |
| 405 nm (Violet) | 525/50 | BV480, BV510, AmCyan, eFluor 506 |
| 405 nm (Violet) | 585/42 | BV570, Pacific Orange |
| 405 nm (Violet) | 605/40 | BV605, Qdot 605 |
| 405 nm (Violet) | 650/50 | BV650, Super Bright 645, Qdot 655 |
| 405 nm (Violet) | 710/50 | BV711, Super Bright 702, Qdot 705 |
| 405 nm (Violet) | 780/60 | BV785, BV786, Qdot 800, Zombie Violet, Live/Dead Violet |
| 488 nm (Blue) | 530/30 | FITC, Alexa Fluor 488, BB515, GFP, CFSE, Zombie Green, Live/Dead Green |
| 488 nm (Blue) | 575/26 | PE (partial excitation), PE-Dazzle 594 (partial) |
| 488 nm (Blue) | 610/20 | PE-CF594 (partial) |
| 488 nm (Blue) | 695/40 | PerCP, PerCP-Cy5.5, PerCP-eFluor 710, BB700 |
| 488 nm (Blue) | 780/60 | PE-Cy5.5 (spillover), PE-Cy7 (spillover), BB755, BB790 |
| 532 nm (Green) | 575/26 | PE |
| 532 nm (Green) | 610/20 | PE-Dazzle 594, PE-CF594, PE-Texas Red, PE-Alexa Fluor 610 |
| 532 nm (Green) | 660/20 | PE-Cy5 |
| 532 nm (Green) | 710/50 | PE-Cy5.5 |
| 532 nm (Green) | 780/60 | PE-Cy7, PE-Vio770 |
| 633 nm (Red) | 660/20 | APC, Alexa Fluor 647, eFluor 660 |
| 633 nm (Red) | 710/50 | APC-R700, Alexa Fluor 700 |
| 633 nm (Red) | 780/60 | APC-Cy7, APC-Alexa 750, APC-eFluor 780, APC/Fire 750, Live/Dead NIR |
Important Notes for Users:
- 488 nm and 532 nm lasers can both excite PE, but 532 nm provides stronger excitation.
- Avoid pairing PE-Cy5 with APC when possible due to spectral overlap.
- Tandem dyes (PE-Cy7, APC-Cy7) require careful handling and compensation.
- Always run single-stained controls for compensation.
- Confirm exact filter configuration with the Flow Core before ordering antibodies.