BD FACSCanto II
A three-laser flow cytometer designed for routine multicolor analysis with improved sensitivity and flexibility compared to entry-level systems.
Best suited for:
- Standard immunophenotyping panels
- Clinical and translational research workflows
- Medium-complexity multicolor experiments
Access: Available for trained users (24/7 self-operated)
Technical highlights:
- 3-laser configuration (405 nm violet, 488 nm blue, 640 nm red)
- Up to 6 fluorescence detectors
- Optimized for routine multicolor analysis with stable performance
Technical specifications
BD FACSCanto II Configuration
| Laser | Detector Filters | Compatible Fluorochromes (BD + other vendors) |
|---|---|---|
| 405 nm (Violet) | 450/50 | BV421, Pacific Blue, eFluor 450 |
| 405 nm (Violet) | 525/50 | BV510, AmCyan, V500 |
| 405 nm (Violet) | 585/42 | BV570, Pacific Orange |
| 405 nm (Violet) | 605/40 | BV605 |
| 405 nm (Violet) | 650/50 | BV650 |
| 405 nm (Violet) | 710/50 | BV711 |
| 405 nm (Violet) | 780/60 | BV785, Zombie Violet |
| 488 nm (Blue) | 530/30 | FITC, Alexa Fluor 488, BB515, GFP, CFSE |
| 488 nm (Blue) | 575/26 | PE |
| 488 nm (Blue) | 610/20 | PE-CF594 |
| 488 nm (Blue) | 695/40 | PerCP, PerCP-Cy5.5, BB700 |
| 488 nm (Blue) | 780/60 | PE-Cy7, BB755 |
| 633 nm (Red) | 660/20 | APC, Alexa Fluor 647, eFluor 660 |
| 633 nm (Red) | 710/50 | APC-R700, Alexa Fluor 700 |
| 633 nm (Red) | 780/60 | APC-Cy7, APC-eFluor 780, Live/Dead NIR |