BD FACSAria II (Cell Sorter)
BD FACSAria II
A high-speed, high-performance cell sorter designed for precise isolation of cell populations under sterile and biosafety-compliant conditions.
Best suited for:
- High-purity and high-yield sorting
- Rare population isolation
- Downstream applications requiring sterile conditions (e.g., culture, sequencing)
Technical Highlights:
- Multi-laser, multi-parameter sorting capability, 4 way sorter
- Aerosol management and biosafety containment
- High-speed sorting with maintained cell viability
Operation: Staff-operated to ensure sample integrity, safety, and reproducibility
BD FACSAria II (Zelda)
A high-speed, high-performance cell sorter designed for precise isolation of cell populations under sterile and biosafety-compliant conditions.
Best suited for:
- High-purity and high-yield sorting
- Rare population isolation
- Downstream applications requiring sterile conditions (e.g., culture, sequencing)
Technical Highlights:
- Multi-laser, multi-parameter sorting capability, 4 way sorter
- Aerosol management and biosafety containment
- High-speed sorting with maintained cell viability
Operation: Staff-operated to ensure sample integrity, safety, and reproducibility
Flow Cytometry Laser and Fluorochrome Compatibility Reference
| Laser | Detector Filter | Compatible Fluorochromes (BD + other vendors) |
|---|---|---|
| 360 nm (UV) | 450/50 | DAPI, Hoechst, Live/Dead Blue |
| 360 nm (UV) | 405 | Indo-1 |
| 360 nm (UV) | 450 | Cascade Blue |
| 405 nm (Violet) | 450/50 | BV421, Pacific Blue, eFluor 450, V450 |
| 405 nm (Violet) | 525/50 | BV510, AmCyan, V500, Qdot 525 |
| 405 nm (Violet) | 585/42 | BV570, Pacific Orange, Krome Orange |
| 405 nm (Violet) | 605/40 | BV605, Qdot 605, eFluor 605 |
| 405 nm (Violet) | 650/50 | BV650, eFluor 650, Qdot 655 |
| 405 nm (Violet) | 710/50 | BV711, Qdot 700 |
| 405 nm (Violet) | 780/60 | BV785, BV786, Zombie Violet, Live/Dead Violet |
| 488 nm (Blue) | 530/30 | FITC, Alexa Fluor 488, BB515, GFP, CFSE |
| 488 nm (Blue) | 575/26 | PE (partial excitation) |
| 488 nm (Blue) | 610/20 | PE-CF594, PE-Alexa 610, PE-Texas Red |
| 488 nm (Blue) | 695/40 | PerCP, PerCP-Cy5.5, PerCP-eFluor 710, BB700 |
| 488 nm (Blue) | 780/60 | PE-Cy7, BB755, BB790 |
| 633 nm (Red) | 660/20 | APC, Alexa Fluor 647, eFluor 660 |
| 633 nm (Red) | 710/50 | APC-R700, Alexa Fluor 700 |
| 633 nm (Red) | 780/60 | APC-Cy7, APC-Alexa 750, APC-eFluor 780, Live/Dead NIR |