Beckman-Coulter Analytical Ultracentrifuge
The AUC is used to apply a strong centrifugal field to solutions of macromolecules. It is equipped with both absorption and interference optics for the detection of the resulting concentration gradients.
Primary Uses
Determination of molar mass, oligomerization state, stoichiometry, association constant.
Wyatt Technologies Dynamic Light Scattering
The DLS illuminates macromolecular solutions with laser light and measures the fluctuations of the scattered light at a fixed (90°) angle.
Primary Uses
Determination of diffusion coefficient, polydispersity; estimation of molar mass.
Wyatt Technologies MALS
This consists of a light-scattering detector and a differential refractive index detector. Laser (or LED) light illuminates the sample as it passes through flow cells. Can be used with DLS.
Primary Uses
Determination of molar mass, hydrodynamic radius, and extinction coefficients.
VP-ITC Isothermal Titration Calorimeter
The VP-ITC injects one solution into another and measures the association heats. It has a stirred-cell volume of 1.4 mL.
Primary Uses
Determination of association constant, enthalpy of interaction, and entropy of interaction.
J-815 Circular Dichroism Spectrometer
The CD Spectrometer illuminates the sample with UV and/or visual radiation and measures the differential absorption of the right- and left-circularly polarized light.
Primary Uses
Estimation of secondary structure content, determinations of protein stability and of association constant of ligands exhibiting CD.
NanoTemper Microscale Thermophoresis
The MST directs an infrared laser onto a capillary that is filled with a fluorescently labeled molecule and an unlabeled binding partner. Changes in how the labeled molecule moves into or out of the heated volume provide insight into the interaction.
Primary Uses
Determination of association constants and other thermo-dynamic information.
Small-Volume Calorimeter
Like the VP-ITC, the small-volume ITC injects one solution into another and measures the heat of interaction. The stirred volume of this calorimeter is 200 microliters.
Primary Uses
Determination of association constant, enthalpy of association, and entropy of association.