The AUC is used to apply a strong centrifugal field to solutions of macromolecules. It is equipped with both absorption and interference optics for the detection of the resulting concentration gradients.
Determination of molar mass, oligomerization state, stoichiometry, association constant.
The DLS illuminates macromolecular solutions with laser light and measures the fluctuations of the scattered light at a fixed (90°) angle.
Determination of diffusion coefficient, polydispersity; estimation of molar mass.
This consists of a light-scattering detector and a differential refractive index detector. Laser (or LED) light illuminates the sample as it passes through flow cells. Can be used with DLS.
Determination of molar mass, hydrodynamic radius, and extinction coefficients.
The VP-ITC injects one solution into another and measures the association heats. It has a stirred-cell volume of 1.4 mL.
Determination of association constant, enthalpy of interaction, and entropy of interaction.
The CD Spectrometer illuminates the sample with UV and/or visual radiation and measures the differential absorption of the right- and left-circularly polarized light.
Estimation of secondary structure content, determinations of protein stability and of association constant of ligands exhibiting CD.
The MST directs an infrared laser onto a capillary that is filled with a fluorescently labeled molecule and an unlabeled binding partner. Changes in how the labeled molecule moves into or out of the heated volume provide insight into the interaction.
Determination of association constants and other thermo-dynamic information.
Like the VP-ITC, the small-volume ITC injects one solution into another and measures the heat of interaction. The stirred volume of this calorimeter is 200 microliters.
Determination of association constant, enthalpy of association, and entropy of association.