Critical Anatomy/IHC

The Histo Pathology Core is based upon the premise that research-oriented histology is about meeting the client investigators' need for critical anatomy.

The lab is well versed in:

• Performing dark-field assisted cut-and-look microtomy to meet comparative anatomy between specimens.
• Matching specific sectioning planes to Kaufman mouse embryo or Paxinos brain anatomy atlases.
• Matching sectioning planes to previously cut slides or anatomy depicted in journal articles.

Achieving specific and comparative anatomy between specimens is important for another of the Core's valuable services, fluorescence and immunoperoxidase protein expression studies. The Core is expanding its IHC repertoire after concluding its long standing, but no longer offered, in-situ hybridization service. 

Note on Protein Expression Studies

While the Core has considerable experience with immunohistochemistry, the lab presently offers IHC on a limited number of antigens.

In the case of an investigator needing staining with an "off-menu" antibody, the Core can facilitate investigators who can commit technical staff for training/performance of the stains. For these collaborative IHC efforts conducted in the lab, the Core can provide an array of antigen retrieval reagents, secondary antibodies, chromagens, and fluorophores.