Fixation Methods & Recipes

To assist client investigators, the Histo Pathology Core provides fixation recipes and procedures for quick reference. The hosted files suggest methods for best quality routine histology as well as methods for preserving antigens and chemical moieties sometimes lost through routine procedures. The listed documents are in MS Word xml format (.docx) and readily downloadable.

Fixation Methods

• Organ Fixation for Paraffin and Frozen Histology
• Transcardial Perfusion

Recipes

• Krebs-Henseleit Cardiac Relaxant
• 4% Paraformaldehyde General Fixative
• Methyl Carnoys Mucin Fixative
• Periodic Acid Glycogen Fixative

Other

• Primer on Collaborative ISH