If a particular chemical series shows poor metabolic stability, knowledge of the specific metabolites formed can aid chemists in the design of more stable analogues. A comparison of the metabolites formed in different species can also aid in the selection of appropriate species for toxicology studies.
The Core has a limited ability to evaluate commonly generated metabolites and to predict the general location of those change based on MS/MS analysis using one of its three triple quadrupole LC-MS/MS systems. More recently, use has been made of a Sciex TripleTOF® 6600 System, a UTSW Biochemistry Department resource, in conjunction with the Sciex MetabolitePilot™ Software package to do more unbiased identification of potential metabolites. Assay conditions and requirements are similar to those needed for determination of metabolic stability.