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Publications from 2010 to 2019


Lopez VA, Park BC, Nowak D, Sreelatha A, Zembek P, Fernandez J, Servage KA, Gradowski M, Hennig J, Tomchick DR, Pawłowski K, Krzymowska M, Tagliabracci VS. "A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity." Cell. 2019 Sep 19;179(1):205-218.e21. doi: 10.1016/j.cell.2019.08.020. Epub 2019 Sep 12. PMID: 31522888; PMCID: PMC6754304

Black MH, Osinski A, Gradowski M, Servage KA, Pawłowski K, Tomchick DR, Tagliabracci VS. "Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases." Science. 2019 May 24;364(6442):787-792. doi: 10.1126/science.aaw7446. PMID: 31123136; PMCID: PMC6767918

Peng W, de Souza Santos M, Li Y, Tomchick DR, Orth K. "High-resolution cryo-EM structures of the E. coli hemolysin ClyA oligomers." PLoS One. 2019 May 2;14(5):e0213423. doi: 10.1371/journal.pone.0213423. PMID: 31048915; PMCID: PMC6497250.

Padavannil A, Sarkar P, Kim SJ, Cagatay T, Jiou J, Brautigam CA, Tomchick DR, Sali A, D'Arcy S, Chook YM. "Importin-9 wraps around the H2A-H2B core to act as nuclear importer and histone chaperone.Elife. 2019 Mar 11;8:e43630. doi: 10.7554/eLife.43630. PMID: 30855230; PMCID: PMC6453568.

Li F, Raczynska JE, Chen Z, Yu H. "Structural Insight into DNA-Dependent Activation of Human Metalloprotease Spartan.Cell Rep. 2019 Mar 19;26(12):3336-3346.e4. doi: 10.1016/j.celrep.2019.02.082. PMID: 30893605.

Endapally S, Frias D, Grzemska M, Gay A, Tomchick DR, Radhakrishnan A. "Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes.Cell. 2019 Feb 21;176(5):1040-1053.e17. doi: 10.1016/j.cell.2018.12.042. Epub 2019 Jan 31. PMID: 30712872; PMCID: PMC6428426.

White J, Dhingra SK, Deng X, El Mazouni F, Lee MCS, Afanador GA, Lawong A, Tomchick DR, Ng CL, Bath J, Rathod PK, Fidock DA, Phillips MA. "Identification and Mechanistic Understanding of Dihydroorotate Dehydrogenase Point Mutations in Plasmodium falciparum that Confer in Vitro Resistance to the Clinical Candidate DSM265." ACS Infect Dis. 2019 Jan 11;5(1):90-101. doi: 10.1021/acsinfecdis.8b00211. Epub 2018 Nov 13. PMID: 30375858; PMCID: PMC6467762.


Maziarz M, Leyme A, Marivin A, Luebbers A, Patel PP, Chen Z, Sprang SR, Garcia-Marcos M. "Atypical activation of the G protein Gαq by the oncogenic mutation Q209P." J Biol Chem. 2018 Dec 21;293(51):19586-19599. doi: 10.1074/jbc.RA118.005291. Epub 2018 Oct 23. PMID: 30352874; PMCID: PMC6314142.

Afanador GA, Tomchick DR, Phillips MA. "Trypanosomatid Deoxyhypusine Synthase Activity Is Dependent on Shared Active-Site Complementation between Pseudoenzyme Paralogs." Structure. 2018 Nov 6;26(11):1499-1512.e5. doi: 10.1016/j.str.2018.07.012. Epub 2018 Sep 6. PMID: 30197036; PMCID: PMC6221947.

Sreelatha A, Yee SS, Lopez VA, Park BC, Kinch LN, Pilch S, Servage KA, Zhang J, Jiou J, Karasiewicz-Urbańska M, Łobocka M, Grishin NV, Orth K, Kucharczyk R, Pawłowski K, Tomchick DR, Tagliabracci VS. "Protein AMPylation by an Evolutionarily Conserved Pseudokinase." Cell. 2018 Oct 18;175(3):809-821.e19. doi: 10.1016/j.cell.2018.08.046. Epub 2018 Sep 27. PMID: 30270044; PMCID: PMC6524645.

Majumdar S, Kim T, Chen Z, Munyoki S, Tso SC, Brautigam CA, Rice LM. "An isolated CLASP TOG domain suppresses microtubule catastrophe and promotes rescue.Mol Biol Cell. 2018 Jun 1;29(11):1359-1375. doi: 10.1091/mbc.E17-12-0748. Epub 2018 Apr 5. PMID: 29851564; PMCID: PMC5994897.

Chen Z, Gutowski S, Sternweis PC. "Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase." Data Brief. 2018 Feb 20;17:356-362. doi: 10.1016/j.dib.2018.01.024. PMID: 29876405; PMCID: PMC5988292.

Dada O, Gutowski S, Brautigam CA, Chen Z, Sternweis PC. "Direct regulation of p190RhoGEF by activated Rho and Rac GTPases." J Struct Biol. 2018 Apr;202(1):13-24. doi: 10.1016/j.jsb.2017.11.014. Epub 2017 Nov 28. PMID: 29196061; PMCID: PMC5835413.


Bae SJ, Ni L, Osinski A, Tomchick DR, Brautigam CA, Luo X. "SAV1 promotes Hippo kinase activation through antagonizing the PP2A phosphatase STRIPAK.Elife. 2017 Oct 24;6:e30278. doi: 10.7554/eLife.30278. PMID: 29063833; PMCID: PMC5663475.

Voleti R, Tomchick DR, Südhof TC, Rizo J. "Exceptionally tight membrane-binding may explain the key role of the synaptotagmin-7 C2 A domain in asynchronous neurotransmitter release." Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):E8518-E8527. doi: 10.1073/pnas.1710708114. Epub 2017 Sep 18. PMID: 28923929; PMCID: PMC5635908.

Brautigam CA, Deka RK, Liu WZ, Tomchick DR, Norgard MV. "Functional clues from the crystal structure of an orphan periplasmic ligand-binding protein from Treponema pallidum." Protein Sci. 2017 Apr;26(4):847-856. doi: 10.1002/pro.3133. Epub 2017 Mar 23. PMID: 28168761; PMCID: PMC5368063.

Xu J, Camacho M, Xu Y, Esser V, Liu X, Trimbuch T, Pan YZ, Ma C, Tomchick DR, Rosenmund C, Rizo J. "Mechanistic insights into neurotransmitter release and presynaptic plasticity from the crystal structure of Munc13-1 C1 C2 BMUN.Elife. 2017 Feb 8;6:e22567. doi: 10.7554/eLife.22567. PMID: 28177287; PMCID: PMC5344669.


Li P., Rivera-Cancel G., Kinch L.N., Salomon D., Tomchick D.R., Grishin N.V., Orth K. "Bile salt receptor complex activates a pathogenic type III secretion system." Elife 5:e15718. PMID: 27377244

Phillips M.A., White K.L., Kokkonda S., Deng X., White J., El Mazouni F., Marsh K.C., Tomchick D.R., Manjalanagara K., Rudra K.R., Wirjanata G., Noviyanti R., Price R.N., Marfurt J., Shackleford D.M., Chiu F.C., Campbell M., Jimenez Diaz M.B., Ferrer-Bazaga S., Angulo-Barturen I., Martínez-Martínez M.S., Lafuente-Monasterio M.J., Kaminsky W., Silue K., Zeeman A.M., Kocken C., Leroy D., Blasco B., Rossignol E., Rueckle T., Matthews D., Burrows J.N., Waterson D., Palmer M.J., Rathod P.K., Charman S.A. "A triazolopyrimidine-based dihydroorotate dehydrogenase inhibitor (DSM421) with improved drug-like properties for treatment and prevention of malaria." ACS Infect Dis. 2:945-957. PMID: 27641613

Kikuchi S., Borek D.M., Otwinowski Z., Tomchick D.R., Yu H. "Crystal structure of the cohesion loader Scc2 and insight into cohesinopathy." Proc Natl Acad Sci USA 113:12444-12449. PMID: 27791135.

Volkov O.A., Kinch L.N., Ariagno C., Deng X., Zhong S., Grishin N.V., Tomchick D.R., Chen Z., Phillips M.A. "Relief of autoinhibition by conformational switch explains enzyme activation by a catalytically dead paralog." Elife 5:e20198. PMID: 27977001

Deka R.K., Brautigam C.A., Liu W.Z., Tomchick D.R., Norgard M.V. "Molecular insights into the enzymatic diversity of flavin-trafficking protein (Ftp; formerly ApbE) in flavoprotein biogenesis in the bacterial periplasm." MicrobiologyOpen 5:21-38. PMID: 26626129

Dodd D.W., Tomchick D.R., Corey D.R., Gagnon K.T. "Pathogenic C9ORF72 antisense repeat RNA forms a double helix with tandem C:C mismatches." Biochemistry 55:1283-6. PMID: 26878348

Ouyang Z., Zheng G., Tomchick D.R., Luo X., Yu H. "Structural basis and IP6 requirement for Pds5-dependent cohesion dynamics." Mol Cell 62:248-59. PMID: 26971492

Kokkonda S., Deng X., White K.L., Coterón J.M., Marco M., de Las Heras L., White J., El Mazouni F., Tomchick D.R., Manjalanagara K., Rudra K.R., Chen G., Morizzi J., Ryan E., Kaminsky W., Leroy D., Martínez-Martínez M.S., Jimenez Diaz M.B., Bazaga S.F., Angulo-Barturen I., Waterson D., Burrows J.N., Matthews D., Charman S.A., Phillips M.A., Rathod P.K. "Tetrahydro-2-napthyl and 2-indanyl triazolopyrimidines targeting Plasmodium falciparum dihydroorotate dehydrogenase display potent and selective antimalarial activity." J Med Chem 59:5416-31. PMID: 27127993

Nguyen K.B., Sreelatha A., Durrant E., Lopez-Garrido J., Muszewska A., Dudkiewicz M., Grynberg M., Yee S.S., Pogliano K., Tomchick D.R., Pawlowski K., Dixon J.E., Tagliabracci V.S. "Phosphorylation of spore coat proteins by a new family of kinases." Proc Natl Acad Sci USA 113:E3482-E3491. PMID: 27185916


Guo Y., Scheuermann T.H., Partch C.L., Tomchick D.R., Gardner, K.H. "Coiled-coil coactivators play a structural role mediating interactions in hypoxia inducible factor heterodimerization." J Biol Chem 290:7707-7721. PMID: 25627682

Deka R.K., Brautigam C.A., Liu W.Z., Tomchick D.R., Norgard M.V. "Evidence for posttranslational protein flavinylation in the syphilis spirochete Treponema pallidum: structural and biochemical insights from the catalytic core of periplasmic flavin-trafficking protein." mBio 6:e00519-15. PMID: 25944861

Brewer K.D., Bacaj T., Cavalli A., Camilloni C., Swarbrick J.D., Liu J., Zhou A., Zhou P., Barlow N., Xu J., Seven A.B., Prinslow E.A., Voleti R., Häussinger D., Bonvin A.M.J.J., Tomchick D.R., Vendruscolo M., Graham B., Südhof T.C., Rizo J. "Dynamic synaptotagmin-1-SNARE complex binding mode in solution." Nat Struct Mol Biol 22:555-64. PMID: 26030874

Phillips M.A., Lotharius J., Marsh K., White J., Dayan A., White K.L., Njoroge J.W., El Mazouni F., Lao Y., Kokkonda S., Tomchick D.R., Deng X., Laird T., Bhatia S.N., March S., Ng C.L., Fidock D.A., Wittlin S., Lafuente-Monasterio M., Benito F.J., Alonso L.M., Martinez M.S., Jimenez-Diaz M.B., Bazaga S.F., Angulo-Barturen I., Haselden J.N., Louttit J., Cui Y., Sridhar A., Zeeman A.M., Kocken C., Sauerwein R., Dechering K., Avery V.M., Duffy S., Delves M., Sinden R., Ruecker A., Wickham K.S., Rochford R., Gahagen J., Iyer L., Riccio E., Mirsalis J., Bathhurst I., Rueckle T., Ding X., Campo B., Leroy D., Rogers M.J., Rathod P.K., Burrows J.N., Charman S.A. "A long-duration dihydroorotate dehydrogenase inhibitor (DSM265) for prevention and treatment of malaria." Sci Transl Med 7:296ra111. PMID: 26180101

Pascoe, H.G., Gutowski, S., Chen, H., Brautigam, C.A., Chen, Z., Sternweis, P.C. and Zhang, X. “Secondary PDZ domain-binding site on class B plexins enhances the affinity for PDZ-RhoGEF.Proc. Natl. Acad. Sci..112 (48): 14852-57.


Fortune D.E., Lin Y.-P., Deka R.K., Groshong A.M., Hagman K.E., Leong J.M., Tomchick D.R., Blevins J.S. "Identification of lysine residues in the Borrelia burgdorferi DbpA adhesin required for murine infection." Infect Immun 82:3186-3198. PMID: 24842928

Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick D.R., Yu H. "Structure of cohesion subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion." Nat Struct Mol Biol 21:864-870. PMID: 25173175

Lin Z., Jia L., Tomchick D.R., Luo X., Yu H. "Substrate-specific activation of the mitotic kinase Bub1 through intramolecular autophosphorylation and kinetochore targeting." Structure 22:1616-1627. PMID: 25308863

Rivera-Cancel G., Ko W.-H., Tomchick D.R., Correa F., Gardner K.H. "Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation." Proc Natl Acad Sci USA 111:17839-44. PMID: 25468971

Hunter, J., Gurbani, D., Ficarro, S.B., Carrasco, M., Lim, S.M., Choi, H.G., Xie, T., Marto, J.A., Chen, Z., Gray, N.S. and Westover, K.D. “In situ Selectivity Profiling and Crystal Structure of SML-8-73-1, an Active Site Inhibitor of Oncogenic K-Ras G12C.” Proc. Natl. Acad. Sci. 111(24): 8895-8900.

Zhang, X., Wu, J., Du, F., Xu, H., Sun, L., Chen, Z., Brautigam, C. A., Zhang, X. and Chen, Z. J. “The cytosolic DNA Sensor cGAS Forms An Oligomeric Complex with DNA and Undergoes Switch-like Conformational Changes in the Activation Loop. Cell Report 6(3): 421-30.


Deka R.K., Brautigam C.A., Liu W.Z., Tomchick D.R., Norgard M.V. "The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis." J Biol Chem 16:11106-11121. PMID: 23447540

Ouyang Z., Zheng G., Song J., Borek D.M., Otwinowski Z., Brautigam C.A., Tomchick D.R., Rankin S., Yu H. "Structure of the human cohesion inhibitor Wapl." Proc Natl Acad Sci USA110: 11355-60. PMID: 23776203

Ni L., Li S., Yu J., Min J., Brautigam C.A., Tomchick D.R., Pan D., Luo X. "Structural basis for autoactivation of human Mst2 kinase and its regulation by RASSF5." Structure 21: 1757-68. PMID: 23972470. PMID: 23972470

Zahm J.A., Padrick S.B., Chen Z., Pak C.W., Yunus A.A., Henry L., Tomchick D.R., Chen Z., Rosen M.K. "The bacterial effector VopL organizes actin into filament-like structures." Cell 155: 423-434. PMID: 24120140

Medina, F., Carter, A., Dada, O., Gutowski, S., Hadas, J., Chen, Z. and Sternweis, P. C. "Activated RhoA Is a Positive Feedback Regulator of the Lbc Family of Rho Guanine Nucleotide Exchange Factor Proteins.” J. Biol Chem 288: 11325-33.


Thompson J.W., Salahudeen A.A., Chollangi S., Ruiz J.C., Brautigam C.A., Makris T.M., Lipscomb J.D., Tomchick D.R., Bruick R.K. "Structural and molecular characterization of the iron-sensing hemerythrin-like domain within F-box and Leucine-rich Repeat Protein 5 (FBXL5)." J Biol Chem 287:7357-7365. PMID: 22253436

Deka R.K., Brautigam C.A., Goldberg M., Schuck P., Tomchick D.R., Norgard M.V. "Structural, bioinformatic, and in vivo analyses of two Treponema pallidum lipoproteins reveal a unique TRAP transporter." J Mol Biol 416:678-696. PMID: 22306465

Matos M.M., Xu Y., Dulubova I., Otwinowski Z., Richardson J.M., Tomchick D.R., Rizo J., Ho A. "Autoinhibition of Mint1 adaptor protein regulates APP binding and processing." Proc Natl Acad Sci USA 109:3802-3807. PMID: 22355143

Kim S., Sun H., Tomchick D.R., Yu H., Luo X. "Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting." Proc Natl Acad Sci USA 109:6549-6554. PMID: 22493223

Brautigam C.A., Deka R.K., Schuck P. Tomchick D.R., Norgard M.V. "Structural and thermodynamic characterization of the interaction between two periplasmic Treponema pallidum lipoproteins that are components of a TPR-protein-associated TRAP transporter (TPAT)." J Mol Biol 420:70-86. PMID: 22504226

Brautigam C.A., Deka R.K., Ouyang Z., Machius M., Knutsen G., Tomchick D.R., Norgard M.V. "Biophysical and bioinformatics analyses implicate the Treponema pallidum Tp34 lipoprotein in transition metal homeostasis." J Bacteriol 194:6771-6781. PMID 23042995

Tian W., Li B., Warrington R., Tomchick D.R., Yu H., Luo X. "Structural analysis of human Cdc20 supports multi-site degron recognition by APC/C." Proc Natl Acad Sci USA 109:18419-24. PMID 23091007

Chen, Z., Guo, L., Hadas, J., Sprang, S. R. and Sternweis, P. C. "Activation of p115-RhoGEF Requires Direct Association of Ga13 and the Dbl-homology Domain." J. Biol Chem 287: 16369-77.


Li W., Ma C., Guan R., Xu Y., Tomchick D.R., Rizo J. "The crystal structure of a Munc13 C-terminal module exhibits a remarkable similarity to vesicle tethering factors." Structure, 19, 1443-1455.

Haines, D.C., Hegde, A., Chen, B., Zhao, W., Bondlela, M., Humphreys, J.M., Mullin, D.A., Tomchick, D.R., Machius, M., Peterson, J.A. "A single active-site mutation of P450BM-3 dramatically enhances substrated binding and rate of product formations." Biochemistry, 50, 8333-8341.

Yu, B., Cheng ,H.C., Brautigam, C.A., Tomchick ,D.R., Rosen, M.K. "Mechanism of actin filament nucleation by the bacteral effector VopL." Nat. Struct. Molec. Biol., 18, 1068-1074.

Padrick, S.B., Doolittle, L.K., Brautigam, C.A., King, D.S., Rosen, M.K. "Arp2/3 complex is bound and activated by two WASP proteins." Proc. Natl. Acad. Sci., 108, E472-E479.

Brautigam, C.A., Wynn, R.M., Chuang, J.L., Naik, M.T., Young, B.B., Huang, T. & Chuang, D.T. "Structural and thermodynamic basis for weak interactions between dihydrolipoamide dehydrogenase and subunit-binding domain of the branched-chain alpha-ketoacid dehydrogenase complex." J. Biol. Chem., 286, 23478-23488.

Kang, J., Chaudhury, J. Dong, H., Brautigam, C.A., & Yu, H. "INCENP-mediated mitotic centromere targeting and Sgo1 binding of HP1 are dispensible for sister-chromatid cohesion in human cells." Mol. Biol. Cell, 22, 1181-1190.

Padrick, S.B. & Brautigam, C.A. "Evaluating the stoichiometry of macromolecular complexes using multisignal sedimentation velocity." Methods, in press.

Brautigam, C.A. "Using Lamm-Equation modeling of sedimentation velocity data to determine the kinetic and thermodynamic properties of macromolecular interactions." Methods, in press.

Hoopman, T.C., Liu, W., Joslin, S.N., Pybus, C., Brautigam, C.A., & Hansen, E.J. "Identification of gene products involved in the oxidative stress response of Moraxella catarrhalis." Infect. Immun., 79, 745-755.

Selyunin, A.S., Sutton, S.E., Weigele, B.A., Reddick, L.E., Orchard, R.C., Bresson, S.M. Tomchick, D.R., & Alto, N.M. "The assembly of a GTPase-kinase signalling complex by a bacterial catalytic scaffold." Nature, 469, 107-111.

Chen, Z., Guo, L., Sprang, S. R. and Sternweis, P. C. "Modulation of a GEF Switch: Autoinhibition of the Intrinsic Guanine Nucleotide Exchange Activity of p115-RhoGEF." Protein Science 20: 107-117.


Dorwart, M.R., Wray, R., Brautigam, C.A., Jiang, Y. & Blount, P. "S. aureus MscL is a pentamer in vivo but of variable stoichiometries in vitro: implications for detergent-solubilized membrane proteins." PLoS Biology, 8, e1000555.

Otomo, T., Tomchick, D.R., Otomo, C, Machius, M., & Rosen, M.K. "Crystal structure of the Formin mDia1 in autoinhibited conformation." PLoS One, 5, e12896.

Xue, M., Craig, T.K., Shin, O.H., Yi, L., Brautigam, C.A., Tomchick, D.R., Sudhof, T.C., Rosenmund, C., & Rizo, J. "Structural and mutational analysis of functional differentiation between synaptotagmins-1 and -7." PLoS One, 5, e12544.

Labandeira-Rey, M., Brautigam, C.A., & Hansen, E.J. "Initial characterization of the CpxRA proteins and the associated regulon in Haemophilus ducreyi." Infect. Immun., 78, 4779-4791.

Padrick, S.B., Deka, R.K., Chuang, J.L., Wynn, R.M., Chuang, D.T., Norgard, M.V., Rosen, M.K., & Brautigam, C.A. "Determination of protein complex stoichiometry through multisignal sedimentation velocity experiments." Anal. Biochem., 407, 89-103.

Deng, X., Lee, J., Michael, A.J., Tomchick, D.R., Goldsmith, E.J., & Phillips, M.A. "Evolution of substrate specificity within a diverse family of b/a-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine." J. Biol. Chem., 285, 25708-25719.

Luong, P., Kinch, L.N, Brautigam, C.A., Grishin, N.V., Tomchick, D.R., & Orth, K. "Kinetic and structural insights into the mechanism of AMPylation by VopS FIC domain." J. Biol. Chem., 285, 20155-20163.

Tian, W., Yu, J., Tomchick, D.R., Pan, D., & Luo, X. "Structural and functional analysis of the YAP-binding domain of human TEAD2." Proc. Natl. Acad. Sci (USA), 107, 7293-7298. [PDF]

Shin O.H., Lu, J. Rhee, J.S., Tomchick, D.R. Pang, Z.P., Wojcik, S.M., Camacho-Preez, M., Brose, N., Machius, M., Rizo, J., Rosenmund, C., & Sudhof, T.C. "Munc13 C2B domain is an activity-dependent Ca2+ regulator of synaptic exocytosis." Nat. Struct. Mol. Biol., 17, 280-288. [PDF]

Yu, B., Martins, I.R., Li, P., Amarasinghe G.K., Umetani, J., Fernandez-Zapico, M.E., Billadeau, D.D., Machius, M., Tomchick, D.R., & Rosen, M.K. "Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1." Cell, 140, 246-256. [PDF]