Flow Cytometry
The flow cytometry facility is a shared resource facility providing cell analysis and sorting to the entire UT Southwestern campus. The facility offers training of investigators, consultation of experimental design, data acquisition, data analysis, cell sorting, and data management. The facility is equipped with eight benchtop analyzers for user operated data acquisition and four sorters which are staff operated. The cytometers allow for up to 18 fluorescent colors capacity on both analyzers and sorters.
Mandatory training by facility staff is required for all users. After training users are granted access to run the benchtop analyzers and utilize analysis computers. Training is $100 for the Scan and Caliburs and $150 for the Canto, CyAn, LSR II, and ImageStream. Training should be scheduled when an experiment is available.
Equipment
| Instrument | Wavelengths | Colors | Hourly Fee |
| Scan | 488nm laser | 3 color | $30 |
| Calibur | 488nm and 635nm lasers | 4 color | $30 |
| CyAn | 488nm, 635nm, 405nm lasers | 9 color | $50 |
| Canto | 488nm, 561nm, 635nm | 8 color | $50 |
| LSR | 488nm, 532nm, 405nm, 635nm lasers | 16 color | $60 |
| ImageStream | 488nm, 634nm lasers | 5 color | $60 |
There is a Moflo and three FACSArias available in the facility for sorting. Facility staff operates the sorters for the user. The sorters are equipped with various combinations of two to five lasers with up to 18 colors available.
| Instrument | Colors | Hourly Fee |
| MoFlo and Arias | Up to 18 | $85 |
Data Analysis
Computers and multiple software packages are available for data analysis free of charge to users.