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A profile on Sergio Cepeda, who researches Vitamin A as a PROVIDES postdoctoral scholar at UT Southwestern.

Nikon CSU-W1 SoRa (NL5.120R) Spinning disk confocal microscope for fixed or live samples Build for speed, allowing visualization of rapid dynamics in live cells and tissues SoRa module for resolution down to 120 nm. Piezo z-drive for fast z-stack acquisition. Fast xy-stage for multi-point acquisition and tiling. Perfect Focus System to keep your sample in focus High acquisition speed ensures better cell viability and reduced photobleaching compared to point-scanning LSM. 405, 445, 488, 514, 561

Dr. Daniela Nicastro has stepped down as Director of the Cryo-EM Facility at UT Southwestern.

The Metabolomics Core Facility focuses on targeted metabolomics (quantitation and validation) and untargeted metabolomics (discovery of metabolites).

Nikon Widefield Epi-scope (NL5.120R) Inverted Nikon EclipseTi with high-quality optics for widefield epifluorescence in fixed and live cells Motorized xy-stage for multi-point acquisition and tiling. Perfect Focus System to keep your sample in focus Excitation filters centered around 350, 405, 445, 488, 514, 555 and 645 nm 10x, 20x, 40x, 60x and 100x objectives with phase or DIC available Andor Zyla 5.5 sCMOS with large 2560x2160px field of view for fluorescence Teledyne Infinity3-6UR color

OMX SR (NL5.120R) 3D structured illumination microscopy (SIM) TIRF SIM 2D PALM/STORM Ring TIRF FRAP, photoactivation Deconvolution Nanopositioning motorized xyz stage Live cell incubation Sign Up Back-to top

Nikon CSU-W1 dual camera (NL5.120R) Spinning disk confocal microscope for fixed or live samples Build for speed, allowing visualization of rapid dynamics in live cells and tissues Can be operated in single camera mode or dual camera mode for true simultaneous 2-channel acquisition. Piezo z-drive with 600 µm travel for fast z-stack acquisition. Fast xy-stage for multi-point acquisition and tiling. Perfect Focus System to keep your sample in focus High acquisition speed ensures better cell

Determination of Intracellular Compound Concentrations Occasionally large differences in compound potency in biochemical versus cell-based assays are noted. While protein binding may account for some of this discrepancy, we have on occasion noted differences in the extent to which different compounds accumulate inside of cells or the extent to which the same compound accumulates in different cells (1). We have developed an intracellular compound accumulation assay to measure the amount of

The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.

Profile on Keisha Hardeman, Ph.D., a postdoctoral scholar who researches Nonalcoholic Fatty Liver Disease in the PROVIDES program at UT Southwestern.