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Special Stains The Histo Pathology Core offers a wide menu of special stains for improving visualization of normal and disease state chemical moieties. Special Stains that the Core performs on a routine schedule include Oil Red-O stain for neutral lipids, Masson's Trichrome stain for collagen/fibrosi…

ChIP-Seq Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP…

Whole Genome Samples are run with the Genomic Tape on the Agilent Tapestation 4200 and their concentrations quantified by fluorometery. One microgram genomic DNA is sonicated using Covaris E220 ultrasonicator targeting 400bp. Libraries are prepared with the KAPA Hyper Prep Kit. Samples are end repair…

In Vitro Metabolic Stability Unless a compound is a pro-drug, good metabolic stability is important for achieving therapeutic concentrations for the sufficient amount of time in vivo. The liver is the most important site of drug metabolism in vivo , although other organs (gastrointestinal tract and l…

Metabolite Profiling If a particular chemical series shows poor metabolic stability, knowledge of the specific metabolites formed can aid chemists in the design of more stable analogues. A comparison of the metabolites formed in different species can also aid in the selection of appropriate species f…

Plasma Protein Binding Plasma protein binding (PPB) plays an important role in the pharmacokinetics (PK) and pharmacodynamics (PD) of a drug. Free drug levels are closely related to the pharmacological effects of a compound as the principal determinant of tissue distribution, cell entry, receptor int…

Eligibility requirements for UT Southwestern’s Radiation Oncology student observerships, open to high school, undergraduate medical students, and more.

UT Southwestern Pediatric Gastrointestinal Pathology provides diagnostic services that utilize Children's Medical Center, Clements University Hospital, and Parkland Hospital.

UT Southwestern Pediatric Liver Pathology provides diagnostic services that utilize Children's Medical Center, Clements University Hospital, and Parkland Hospital.

UT Southwestern Perinatal and Placental Pathology group provides diagnostic services that utilizes Clements University Hospital, Parkland Hospital, and Children's Medical Center .