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UTSW study links gene mutation to learning deficits in “Clueless” mice

Joseph Takahashi, Ph.D.

DALLAS – August 25, 2022 – A single mutation in a gene, Kcnc3, which encodes a potassium channel in neurons, causes learning deficits in mice, UT Southwestern researchers report in a new study in PNAS. The novel mutation decreases the activity of neurons in the hippocampus, the area of the brain important for learning and memory, and highlights a new role for potassium channels.

“Learning and memory are very complex at the genetic level. Unbiased searches for genes underlying learning and memory have not been successfully conducted in mice before,” said Joseph Takahashi, Ph.D., Professor and Chair of Neuroscience at UT Southwestern and a Howard Hughes Medical Institute Investigator.

The discovery of the Kcnc3 mutation came out of an extraordinary effort by Dr. Takahashi and colleagues to conduct a large-scale mutagenesis screen in mice. Using a highly potent mutagen called ENU, the researchers induced random mutations in the mouse genome. The progeny of ENU-treated mice were then screened for neural and behavioral traits that could be mapped to specific genes to identify the causal mutation. This approach to unbiased gene discovery is called forward genetics.

From the mutagenesis screen, Dr. Takahashi and his team isolated a mutant mouse with spatial learning defects, which they named Clueless. In fear-conditioning tests, the mutants exhibited reduced freezing (a natural fear response in mice) as well as defects in long-term and short-term memory. The defects in Clueless mice mapped to a mutation in the Kcnc3 gene, which encodes a subunit of a special type of potassium channel called a voltage-gated potassium channel.

This is the first study to implicate Kcnc3 in learning, explained Dr. Takahashi, an investigator in UTSW’s Peter O’Donnell Jr. Brain Institute. The full Kcnc3 knockout mouse model shows only mild gait issues with no defects in learning and memory, which could be due to developmental compensation or functional redundancy with other voltage-gated potassium channel subunits. Future research will help to answer some of these outstanding questions.

Dr. Takahashi said the findings underscore the power of applying forward genetics to gene discovery and could lead to potential new targets for therapy in learning and memory as well as other disease areas.

Dr. Takahashi holds the Loyd B. Sands Distinguished Chair in Neuroscience.

About UT Southwestern Medical Center

UT Southwestern, one of the nation’s premier academic medical centers, integrates pioneering biomedical research with exceptional clinical care and education. The institution’s faculty has received six Nobel Prizes, and includes 26 members of the National Academy of Sciences, 17 members of the National Academy of Medicine, and 14 Howard Hughes Medical Institute Investigators. The full-time faculty of more than 2,900 is responsible for groundbreaking medical advances and is committed to translating science-driven research quickly to new clinical treatments. UT Southwestern physicians provide care in more than 80 specialties to more than 100,000 hospitalized patients, more than 360,000 emergency room cases, and oversee nearly 4 million outpatient visits a year.