NGS Applications

Whole Genome

Samples are run on a 1.2% agarose gel and their concentrations quantified by fluorometry. 1 µg genomic DNA is sonicated using Covaris S2 ultrasonicator and Libraries prepared with the KAPA High Throµghput Library Preparation Kit. Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP beads, then validated on the Agilent 2100 Bioanalyzer. Ampure XP beads, then checked on the Agilent 2100 Bioanalyzer to ensure the library is the expected size. Samples are quantified by Qubit before being normalized and pooled, then run on the Illumina HiSeq 2500 using PE100 SBS v3 reagents.

Current Protocol:

KAPA HTP Library Preparation Kit