Services

Microbial whole genome sequencing

Assay-overview

We provide services to sequence microbial whole genome from bacterial culture or clinical isolates. Customer can submit either extracted DNA or original sample. We input 250-500ng of high complexity genomic DNA and generate sequencing libraries using Covaris 220 sonication or KAPA Biosystem enzyme based fragmentation method. Genomic fragments of 300-500bp size are ligated with illumina recommended adapters containing unique barcodes or Unique Molecular Identifiers (UMIs) from IDT.  KAPA Biosystem library prep kits are used to generate high quality libraries. For example: 24 isolates of Mycobacterium tuberculosis pathogen (approx. genome size 5MB) can be sequenced at 100X coverage on MiSeqDX flow cell using 300PE protocol Or 48 isolates together at 50X coverage. We sequence all kinds of microbial samples from research or clinical studies i.e. E. coli, Staph. aureus, M.tb. NTM, etc.

Example of work flow: