Faculty Resources Employee Resources In Vitro Metabolic Stability Unless a compound is a pro-drug, good metabolic stability is important for achieving therapeutic concentrations for the sufficient amount of time in vivo. The liver is the most important site of drug metabolism in vivo , although other organs (gastrointestinal tract and lung, among others) may play a role. The Core can assess metabolic stability in a variety of different systems, including microsomes, hepatocytes, S9 fractions

Faculty Resources Employee Resources Aqueous Solubility Determination Poor aqueous solubility can limit the ability to develop lead compounds as in vivo probes and therapeutics. Aqueous solubility affects absorption, formulation and ability of a compound to be delivered in sufficient concentrations to be active, and even in vitro activity. The Core offers two types of assays: Thermodynamic (or Equilibrium) Solubility Measures solubility of a compound as a saturated solution in equilibrium

Genaro Hernandez, Ph.D., a postdoctoral scholar, is profiled in a look at his research in human pseudokinases in the PROVIDES program at UT Southwestern.

The McDermott Center Human Genetics Clinical Lab is dedicated to help accelerate the pace of human genetic research by providing skilled assistance with collecting and storing biologic specimens.

Policies and guidance help ensure compliance with University, state, and federal regulations through education and collaboration with the research community.

Policies and guidance help ensure compliance with University, state, and federal regulations through education and collaboration with the research community.

Faculty Resources Employee Resources Small RNA-Seq Current Protocol NEBNext Small RNA Past Protocol TruSeq Small RNA Sample Preparation Kit Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

Faculty Resources Employee Resources mRNA-Seq Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorimeter is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded mRNA Library Prep Kit from Illumina. Poly-A RNA is purified and fragmented before strand specific cDNA synthesis. cDNA are then

Faculty Resources Employee Resources Whole Transcriptome Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorometer is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina. Total RNA is depleted of its rRNA and fragmented before strand-specific

Faculty Resources Employee Resources Exome Capture Current Protocol Twist Past Protocols SureSelect XT v4 Sample Preparation Guide Instructions for Performing the Nextera Rapid Capture Protocol TruSeq Sample Enrichment Guide Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top