As we commemorate National Postdoc Appreciation Week (NPAW), we shine a spotlight on a sampling of our postdocs and their experiences as researchers at UT Southwestern.

Here is how to contact the Small Animal Imaging Resource (SAIR) at UT Southwestern.

Learn about the advisory committee for the Small Animal Imaging Resource at UT Southwestern.

Exome Capture Current Protocol Twist Past Protocols SureSelect XT v4 Sample Preparation Guide Instructions for Performing the Nextera Rapid Capture Protocol TruSeq Sample Enrichment Guide Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

ChIP-Seq Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP beads, then checked on the Agilent 2100 Bioanalyzer to ensure the library is the expected size. Samples are quantified by Qubit before being normalized and pooled, then run on the Illumina HiSeq

Small RNA-Seq Current Protocol NEBNext Small RNA Past Protocol TruSeq Small RNA Sample Preparation Kit Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

Sequencing Our facility is equipped with one Illumina NextSeq 2000, three NextSeq 500s, and one Miseq System. The NextSeq 2000 offers three flowcells: Flowcell # of reads Read Lengths P1 100 M 300 Cycles P2 400 M 100, 200 and 300 Cycles P3 1.2 B 50, 100, 200 and 300 Cycles The NextSeq 500 offers 5 flowcells: Flowcell # of reads Kit Capacity 75 High Output 400 M 75 Cycles 150 Mid Output 130 M 150 Cycles 150 High Output 400 M 150 Cycles 300 Mid Output 130 M 300 Cycles 300 High Output 400 M 300

Contact Us Core Director Noelle S. Williams, Ph.D. Director Professor in Biochemistry Education Ph.D., Microbiology – University of Virginia, Charlottesville, VA B.S., Biology – Trinity University, San Antonio, TX Contact Information Location: L4.244 Office Phone: 214-648-5072 Lab Phone: 214-648-0348 Fax: 214-648-8856 Lab Location: L4.244 Shipping Address Noelle Williams Southwestern Medical Center Central Supply Warehouse, XA100 2929 N. Stemmons Freeway Dallas, TX 75247-9056 Attn: Room L4.244

Publications Recent publications (core staff in bold) Wang W, Ranjan A, Zhang W, Liang Q, MacMillan KS, Chapman K, Wang X , Chandrasekaran P, Williams NS , Rosenbaum DM, De Brabander JK. Novel orexin receptor agonists based on arene- or pyridine-fused 1,3-dihydro-2H-imidazole-2-imines. Bioorg Med Chem Lett . 2024 Feb 1; 99 : 129624 . PubMed PMID: 38272190 . Prakasam G, Mishra A, Christie A, Miyata J, Carrillo D, Tcheuyap VT, Ye H, Do QN, Wang Y, Reig Torras O, Butti R, Zhong H, Gagan J, Jones KB

Pricing & Services The Preclinical Pharmacology Core offers a range of services associated with preclinical drug development and pharmacokinetics on a fee-for-service or collaborative grant-funded basis. See list at left for details of our services. Assay costs vary with the complexity of the study but are built based on prices from the attached ala carte menu which is shown below. Please inquire with the Core director regarding pricing for your particular project. New projects require