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To wrap up the journey, postdocs have several final steps to follow.

Regardless of your prior NMR experience, users who are new to this facility must complete a formal training with the facility manager on an instrument before they are allowed to operate on that instrument, including inserting samples in the spinners or placing samples in the sample tray or on top of

All users must complete the MR safety I and II training and sign and submit the MR Screening Form and the Biochemistry NMR Safety Form before they are permitted to enter the magnet rooms or request NMR training. There will be no exception to this rule. Anyone who let an unauthorized person into a

Leica Stellaris Falcon 8 (NL5.120R) Laser Scanning confocal microscope for fixed or live samples The Falcon 8 module with tauSense technology is a fully integrated solution for Fluorescence Lifetime Imaging (FLIM). 405 nm laser + White light laser (WLL) : e xcite each fluorophore by choosing any wavelength between 440 nm and 790 nm. 5 detectors enabling the detection of faint signals from 400 nm to 850 nm. 8 kHz tandem galvo scanners, switchable between resonant and non-resonant mode Piezo z

Discover the research systems to use when submitting a proposal, executing a contract, managing an award, and more.

mRNA-Seq Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorimeter is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded mRNA Library Prep Kit from Illumina. Poly-A RNA is purified and fragmented before strand specific cDNA synthesis. cDNA are then a-tailed and indexed adapters are

Small RNA-Seq Current Protocol NEBNext Small RNA Past Protocol TruSeq Small RNA Sample Preparation Kit Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

Exome Capture Current Protocol Twist Past Protocols SureSelect XT v4 Sample Preparation Guide Instructions for Performing the Nextera Rapid Capture Protocol TruSeq Sample Enrichment Guide Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

ChIP-Seq Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP beads, then checked on the Agilent 2100 Bioanalyzer to ensure the library is the expected size. Samples are quantified by Qubit before being normalized and pooled, then run on the Illumina HiSeq

Contact Dr. Feng Li about the Nuclear Magnetic Resonance Core facility