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Nikon Widefield Epi-scope (NL5.120R) Inverted Nikon EclipseTi with high-quality optics for widefield epifluorescence in fixed and live cells Motorized xy-stage for multi-point acquisition and tiling. Perfect Focus System to keep your sample in focus Excitation filters centered around 350, 405, 445, 488, 514, 555 and 645 nm 10x, 20x, 40x, 60x and 100x objectives with phase or DIC available Andor Zyla 5.5 sCMOS with large 2560x2160px field of view for fluorescence Teledyne Infinity3-6UR color

Subcutaneous Human Tumor Xenografts The Preclinical Pharmacology Core has data and experience with testing efficacy of compounds in a modest number of human tumor cell lines implanted as subcutaneous xenografts. Please inquire for availability of data with your cell line of interest. New models will be developed upon demand. Request Services Register or Log in to iLabs Note that new projects require consultation with the Core Director and completion of the Compound Submission Form . Related

Determination of Intracellular Compound Concentrations Occasionally large differences in compound potency in biochemical versus cell-based assays are noted. While protein binding may account for some of this discrepancy, we have on occasion noted differences in the extent to which different compounds accumulate inside of cells or the extent to which the same compound accumulates in different cells (1). We have developed an intracellular compound accumulation assay to measure the amount of

(Photo credit: Getty Images) DALLAS – April 15, 2024 – Women who take oral contraceptives may be significantly less likely to experience certain musculoskeletal injuries than women who do not take the drugs or men, according to a study by UT Southwestern Medical Center. The findings, published in Medicine & Science in Sports & Exercise , increase the understanding of the potential protective benefits of stable hormone levels as well as possible concerns about hormone imbalances and

Client Microscopy Facilities The Histo Pathology Core has microscopy facilities for clients to review and document completed histology slides, as well as to document wet tissues and cell culture preps that augment their histopathology. Self-Service Microscopy by Reservation These instruments, located in the Core's Tissue Handling Lab (NB11.204), are offered on a self-service, reservation basis: Leica DM2000 Upright Compound Research Photomicroscope Bright-Field, Dark-Field, Epifluorescence

Routine Histology The Histo Pathology Core offers routine histology services for workup of specimens as paraffin or frozen. Routine histology services offered include: Paraffin Processing and Embedding: Automated, Manual, Chromagen Cryoembedding: TFM, OCT, or Gum Tragacanth Paraffin Sectioning: H&Es and/or unstained slides cut to needed anatomy Cryosectioning: H&Es and/or unstained slides cut to needed anatomy Additional Levels: Ribbon, Interval, Additional Block, Serials Separate Slide Special

Histopath Consultation The Histo Pathology Core offers histopathology consultation by appointment with the lab's Director, Bret Evers, Assistant Professor, and board-certified neuropathologist. Dr. Evers is expert in the analysis of murine phenotypes, with a strong emphasis in comparative pathology and developmental biology. He is an excellent collaborator and teacher. His histopathologic expertise: Helps characterize mutant mouse phenotypes. Helps develop experimental models of human disease

Special Stains The Histo Pathology Core offers a wide menu of special stains for improving visualization of normal and disease state chemical moieties. Special Stains that the Core performs on a routine schedule include Oil Red-O stain for neutral lipids, Masson's Trichrome stain for collagen/fibrosis, Periodic-Acid Schiff for sugars and basement membrane, as well as Hart's variation of the Verhoeff Van Gieson's technique for elastin. Optimum staining for some substances requires a specific

Older Publications for the Preclinical Pharmacology Core at UT Southwestern

Dr. Daniela Nicastro has stepped down as Director of the Cryo-EM Facility at UT Southwestern.