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The overall goal of the NEI Center Core Grant for Vision Research is to enhance the capabilities of vision research scientists by facilitating easy access to equipment, training and technical support, which expands experimental capabilities, results in more cost-effective and time-efficient research

Discover the research systems to use when submitting a proposal, executing a contract, managing an award, and more.

Roles & Responsibilities Principal Investigator (PI) and Department Research Staff Individual designated to oversee project sponsored by a grant award. They are responsible for the proper conduct of a research project. Faculty in full-time positions (instructor, assistant professor, associate professor, and professor) automatically have PI status. Departmental research staff assists PIs with administrative functions of submitting proposals and managing awards. This role is engaged with these

Routine Histology The Histo Pathology Core offers routine histology services for workup of specimens as paraffin or frozen. Routine histology services offered include: Paraffin Processing and Embedding: Automated, Manual, Chromagen Cryoembedding: TFM, OCT, or Gum Tragacanth Paraffin Sectioning: H&Es and/or unstained slides cut to needed anatomy Cryosectioning: H&Es and/or unstained slides cut to needed anatomy Additional Levels: Ribbon, Interval, Additional Block, Serials Separate Slide Special

Client Microscopy Facilities The Histo Pathology Core has microscopy facilities for clients to review and document completed histology slides, as well as to document wet tissues and cell culture preps that augment their histopathology. Self-Service Microscopy by Reservation These instruments, located in the Core's Tissue Handling Lab (NB11.204), are offered on a self-service, reservation basis: Leica DM2000 Upright Compound Research Photomicroscope Bright-Field, Dark-Field, Epifluorescence

Zeiss SteREO Discovery (NL7.110M) Zeiss motorized stereo microscope with filters for GFP, RFP, and CY5 GFP LP available for simultaneous GFP and transmitted-light imaging Automated collection of z-stacks with extended focus option Transillumination and gooseneck illumination for bright field imaging Axiocam MRm B&W (cooled) and Axiocam MRc5 color cameras. Axiovision software for microscope control Good for screening and documentation of whole-mount flies, worms, mice, organs and tissue sections

Zeiss LSM 780 Inverted (NL5.120N) Visible laser lines: 405, 458, 488, 514, 561, 594, and 633 nm for conventional confocal applications. Spectral detection for optimized CFP/YFP FRET and spectral unmixing of closely overlapping fluorophores (e.g. GFP and YFP, multiplexed Qdots). Tunable NIR laser for multiphoton excitation from 690 to 1040 nm, including second harmonic generation (SHG) imaging of unstained collagen. Two photon excitation of UV fluorophores down to 350 nm (e.g. NADH). FRAP

The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.

Plasma Protein Binding Plasma protein binding (PPB) plays an important role in the pharmacokinetics (PK) and pharmacodynamics (PD) of a drug. Free drug levels are closely related to the pharmacological effects of a compound as the principal determinant of tissue distribution, cell entry, receptor interactions, and availability for elimination. At the early stages of drug development, PPB experiments advance understanding of ADME (absorption, distribution, metabolism, excretion) properties to aid

Older Publications for the Preclinical Pharmacology Core at UT Southwestern