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mRNA-Seq Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorimeter is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then …

Mate-Paired Samples are run on a 1.2% agarose gel and their concentrations quantified by fluorometry. Four µg of genomic DNA are processed using the Nextera Mate Pair Sample Preparation Kit from Illumina. Specifically, the Gel-Plus pathway is followed. Samples are first tagmented then subjected to st…

The U.S. Department of State has released the October 2025 Visa Bulletin (VB), which outlines priority dates for green card applicants.

Anatomic Pathology The Histo Pathology Core offers anatomic pathology services on an as needed basis to train clients as well as facilitate morphologic and histologic endpoints in large terminal studies with mice. The Core provides expert training in the following procedures with mice: Transcardial W…

ChIP-Seq Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP…

Specialized Techniques The Histo Pathology Core offers services that are irregular offshoots of histology, and usually only performed in individual academic or pharmaceutical research lab settings. Services, such as Laser Microdissection and others listed on this page, are technically demanding and s…

Nikon CSU-W1 SoRa (NL5.120R) Spinning disk confocal microscope for fixed or live samples Build for speed, allowing visualization of rapid dynamics in live cells and tissues SoRa module for resolution down to 120 nm. Piezo z-drive for fast z-stack acquisition. Fast xy-stage for multi-point acquisition…

OMX SR (NL5.120R) 3D structured illumination microscopy (SIM) TIRF SIM 2D PALM/STORM Ring TIRF FRAP, photoactivation Deconvolution Nanopositioning motorized xyz stage Live cell incubation Sign Up Back-to top

Whole Transcriptome Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorometer is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated R…

The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.