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Charging Policy What is our policy for charging users? The Proteomics Core has a simple charging policy: No samples will be prepared or run until the information has been entered into our sample submission site. We charge for every sample run , regardless of whether an expected result is obtained. Th…

Price List Analyses are charged per sample submitted. Any gel band or slice must be 1 cm or less of the gel, and must be diced into ~1mm cubes. Gel slices that are not diced or that are minced too fine may be sent back. We charge for all samples , whether or not a result is obtained. The only excepti…

Co-IP Experiments Introduction One of the most common types of experiment submitted for analysis in the Proteomics Core is the Co-IP experiment. An antibody against endogenous or tagged protein is used to pull down a protein of interest. The goal of the experiment is to see what comes down with that …

General Limitations MS proteomics is a powerful tool for the analysis of protein samples, but there are some limits to the techniques we employ in the Proteomics Core. Knowing the limitations of the services we provide can help to ensure your experiment is a success, and you do not incur costs for un…

Silac Quantitation Introduction Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a powerful technique for comparing protein (and peptide) amounts between two or three conditions. Developed in the lab of Matthias Mann it is one of the most widely used labeled quantitation methods in p…

Intact Mass What do we use to measure the mass of intact proteins? We do LC/MS analysis of intact proteins using a Q-TOF mass spectrometer with electrospray ionization. This means that samples must be free of detergents. An ideal solvent is 5% acetonitrile in water containing 1-5% formic acid or acet…

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Cutting Gels Introduction Many proteomics samples are well suited for SDS-PAGE, including protein identification and PTM identification. We recognize that a gel approach may not be compatible with all samples, so please contact us if you have concerns about running your sample into a gel. We require …

Gel Band ID Introduction Identification of proteins from visible bands excised from Coomassie or silver-stained gels using mass-spectrometry is generally very successful. Almost all visible Coomassie bands contain enough protein for MS identification. Most silver-stain bands can be identified on our …

PTM Identification Introduction The presence or absence of post-translational modifications (PTMs), such as phosphorylation, on a protein are often as interesting as the presence of absence of the protein itself. The PTM ID service offers identification of PTMs on a protein from a purified protein or…