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Exome Capture - Next Generation Sequencing Core - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/next-generation-sequencing/ngs-applications/exome-capture.html
Exome Capture Current Protocol Twist Past Protocols SureSelect XT v4 Sample Preparation Guide Instructions for Performing the Nextera Rapid Capture Protocol TruSeq Sample Enrichment Guide Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more informat…
mRNA-Seq - Next Generation Sequencing Core - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/next-generation-sequencing/ngs-applications/mrna-seq.html
mRNA-Seq Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorimeter is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then …
Routine Histology - Services - Histo Pathology Core - EDU - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/histo-pathology/services/routine-histology.html
Routine Histology The Histo Pathology Core offers routine histology services for workup of specimens as paraffin or frozen. Routine histology services offered include: Paraffin Processing and Embedding: Automated, Manual, Chromagen Cryoembedding: TFM, OCT, or Gum Tragacanth Paraffin Sectioning: H&Es …
Sign-up and Cancellation Policies: Electron Microscopy Core Facility - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/emcf/policies/sign-up-and-cancellation.html
The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.
Mate-Paired - Next Generation Sequencing Core - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/next-generation-sequencing/ngs-applications/mate-paired.html
Mate-Paired Samples are run on a 1.2% agarose gel and their concentrations quantified by fluorometry. Four µg of genomic DNA are processed using the Nextera Mate Pair Sample Preparation Kit from Illumina. Specifically, the Gel-Plus pathway is followed. Samples are first tagmented then subjected to st…
Aqueous Solubility Determination: Preclinical Pharmacology Core Lab - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/preclinical-pharmacology/pricing-and-services/aqueous-solubility-determination.html
Aqueous Solubility Determination Poor aqueous solubility can limit the ability to develop lead compounds as in vivo probes and therapeutics. Aqueous solubility affects absorption, formulation and ability of a compound to be delivered in sufficient concentrations to be active, and even in vitro activi…
Histopath Consultation - Services - Histo Pathology Core - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/histo-pathology/services/histopath-consultation.html
Histopath Consultation The Histo Pathology Core offers histopathology consultation by appointment with the lab's Director, Bret Evers, Assistant Professor, and board-certified Neuropathologist and Ophthalmic Pathologist Dr. Evers is expert in the analysis of murine phenotypes, with a strong emphasis …
Zeiss SteREO Discovery: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/quantitative-light-microscopy/equipment-fees-and-services/zeiss-stereo-discovery.html
Zeiss SteREO Discovery (NL7.110M) Zeiss motorized stereo microscope with filters for GFP, RFP, and CY5 GFP LP available for simultaneous GFP and transmitted-light imaging Automated collection of z-stacks with extended focus option Transillumination and gooseneck illumination for bright field imaging …
Zeiss LSM 780 Inverted: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/quantitative-light-microscopy/equipment-fees-and-services/zeiss-lsm-780-inverted.html
Zeiss LSM 780 Inverted (NL5.120N) Visible laser lines: 405, 458, 488, 514, 561, 594, and 633 nm for conventional confocal applications. Spectral detection for optimized CFP/YFP FRET and spectral unmixing of closely overlapping fluorophores (e.g. GFP and YFP, multiplexed Qdots). Tunable NIR laser for …
Data Policy: Electron Microscopy Core Facility - UT Southwestern, Dallas, Texas
https://www.utsouthwestern.edu/research/core-facilities/emcf/policies/data-policy.html
The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.