The DNA Genotyping Core facility performs allelic discrimination using an Applied Biosystems 7900HT real-time PCR instrument and the TaqMan Assay. 


The cost of each SNP assay is $0.30 plus the cost of the Life Technologies® probes. The cost of the probes should be around $0.50 per sample. 

There are several options regarding the ordering of the TaqMan probes from Life Technologies®. Probes can be ordered from Life Technologies's® extensive list of predesigned probes (Assays-By-Design) or Life Technologies® can design and test probes (Assays-On-Demand) if your SNP is not included in this list.

A third option is to design and order the probes yourself. Each method has associated benefits and costs.