See Dr. Yu's published work.

Skip to main content Search Labs A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z Lab Name or Principal Investigator Centers/Institutes Advanced Imaging Research Center Cecil H. and Ida Green Center for Reproductive Biology Sciences Center for Alzheimer's and Neurodegenerative Diseases Center for Hypothalamic Research Center for Organogenesis Research and Trauma Center for the Genetics of Host Defense Charles and Jane Pak Center for Mineral

Meet the TRUST Lab Translational Research Collaboration

1 Joseph Martin Ready Department of Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Dallas, TX 75390-9038 (214) 648-0313 joseph.ready@utsouthwestern.edu Employment 2012 – Professor, UT Southwestern 2009 – Director, Medicinal Chemistry Laboratory, UT Southwestern 2007 – 2012 Associate Professor, UT Southwestern 2007 – Chair, chemistry training track, UT Southwestern 2003 – 2007 Assistant Professor, UT Southwestern and

Eugene McDermott Center for Human Growth and Development Southwestern Medical School 5323 Harry Hines Blvd. / Dallas, Texas 75390-8591 (214) 648-1695 Fax (214) 648-1666 Email: chao.xing@utsouthwestern.edu Computational Biologist I The laboratories of Dr. Ralph DeBerardinis at Children’s Research Institute (https://cri.utsw.edu/scientists/ralph-deberardinis-laboratory/) and Dr. Chao Xing at McDermott Center for Human Growth and Development (https://labs.utsouthwestern.edu

Bu cc al B ru sh Gentra Puregene Handbook 06/201128 Protocol: DNA Purification from a Buccal Brush Using the Gentra Puregene Buccal Cell Kit This protocol is for purification of genomic DNA from 1 buccal brush using the Gentra Puregene Buccal Cell Kit. Things to do before starting � Preheat water baths to 55°C for use in step 3b and 65°C for use in steps 3a and 17 of the procedure. � Optional: Preheat water bath to 37°C for use in step 5 of the procedure. Procedure 1. To collect

1 updated May 2025 FRDA fibroblasts Short alleles < 499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual nd - not determined Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele 2 4 M 30 11 597 848 21 M 35 8 622 738 50 M 33 13 353 616 66 M 70 41 110 590 68 F 21 7 570 1200 86 F 30 18 367 780 88 F 50 16 520 520 90 F 29 7 556 556

1 updated May 2025 Napierala Lab protocol for human skin fibroblast culture Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Dimethyl sulfoxide (DMSO) Sigma D8418-100ML Fibroblast Medium recipe

Didactic Questions 1. Epimysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 2. Perimysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 3. Endomysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 4. Fascicles are A. groupings of