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1 Napierala Lab protocol for culturing human iPSCs (May 2022) For details, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSR™1” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number BD MATRIGEL HESC-QUALIFIED Fisher Scientific 08-774-552 mTeSRTM1 Complete Kit STEMCELL Technologies 85850 Cryostor CS10 STEMCELL Technologies 07930 Dispase 1U/mL, 100mL STEMCELL Technologies 07923 Stemolecule Y27632 Stemgent 04-0012

1 Sendai virus reprogrammed iPSC Lines Line Clone# Sex GAA repeat number in fibroblasts GAA repeat number in iPSCs Earliest passage Karyotype data Karyotype passage 1 CTRL 3348 6 M Control size Control size P5 Yes, normal P11 2 CTRL 7522 5 F Control size Control size P9 Yes, normal P15 3 CTRL 6718 1 M Control size Control size P2 Yes, normal P4 4 CTRL 6718 6 M Control size Control size P2 Yes, normal P4 5 CTRL 6719 1 F Control size Control size

1 Napierala Lab protocol for human skin fibroblast culture (May 2022) Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Fibroblast Medium recipe: DMEM, high glucose 15% fetal bovine serum (FBS

RESEARCH ON SYMPTOM MONITORING AND PREVENTIVE COGNITIVE THERAPY FOR PERINATAL DEPRESSION The Department of Psychiatry at the University of Texas Southwestern Medical Center is conducting research sponsored by the National Institute of Mental Health (NIMH) on perinatal depression, aiming to develop a treatment program for conceiving or pregnant women at risk for depressive relapse and recurrence. We are recruiting women with a history of depression who are planning to become

DNA Purifi cation Protocol for 4 ml Saliva Samples Cell Lysis 1. Incubate Oragene•DNA/Saliva samples at 50ºC in a water incubator for a minimum of 1 hour or in an air incubator for a minimum of 2 hours. 2. Transfer 4 ml lysate sample (2 ml saliva plus 2 ml Oragene•DNA-preserving solution) to a 15 or 50 ml centrifuge tube. 3. Add 1 ml Cell Lysis Solution and 25 μl Gentra RNase A Solution (4 mg/ml). Vortex on high speed for 10 seconds to mix sample and incubate 10 minutes at room

December 26, 2016 Chen Lab Weekly Meeting Schedule Wednesday at 9 am (unless indicated otherwise), NA6.214 1/4/17 Chee-Kwee & Jueqi 1/19/17 (Thursday) Mingjian & Shuiqing (NA8.218) 1/24/17 (Tuesday) Junyao & Seoyun (NA8.218) 2/3/17 (Friday) Youtong & Fenghe 2/8/17 Nicole & Philip 2/15/17 Xiang G & Xiang C 2/24/17 (Friday) Ming & Hanze W 3/1/17 Conggang & Hua 3/8/17 Peiqing & Hui Y 3/15/17 Xiaojun & Junjiao 3/24/17 (Friday

1 FRDA fibroblasts Short alleles <499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele 2 4 M 30 11 597 848 21 M 35 8 622 738 50 M 33 13 353 616 66 M 70 41 110 590 68 F 21 7 570 1200 86 F 30 18 367 780 88 F 50 16 520 520 90 F 29 7 556 556 90B F 33 7 570 570 130 M 56 41

Chen Lab Journal Club The Last Friday of every month, 4:20PM at NA6 small conference room. Date 09.15. Mingjian 10.15. Haocheng 11.15. Seoyun 01.16. Peiqing 02.16. Hui Y. 03.16. Conggang 04.16. Youtong 05.16. Daxing 06.16. Philip 07.16. Tuo 08.16. Hua

Postdoctoral Position in Neuroimaging A postdoctoral training position is available in the BioMedical Imaging Research Lab (BiMIR) led by Dr. Jiaen Liu in the Advanced Imaging Research Center (AIRC) at UT Southwestern Medical Center to develop advanced high resolution MRI methods for neuroimaging applications. BiMIR has several exciting projects related to novel MRI acquisition and reconstruction, ultrahigh-field MRI (≥7 T), quantitative MRI (T2, T2* and MT) for myelin and iron

1 updated May 2025 Napierala Lab protocol for culturing human iPSCs For additional details and recommendations, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSRTM Plus” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number Corning™ Matrigel™ hESC-Qualified Matrix Fisher Scientific 08-774-552 mTeSRTM Plus Complete Kit STEMCELL Technologies 100-0276 CryoStor® CS10 STEMCELL Technologies 07930 Dispase 1U/mL, 100mL