1 Napierala Lab protocol for culturing human iPSCs (May 2022) For details, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSR™1” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number BD MATRIGEL HESC-QUALIFIED Fisher Scientific 08-774-552 mTeSRTM1 Complete Kit STEMCELL Technologies 85850 Cryostor CS10 STEMCELL Technologies 07930 Dispase 1U/mL, 100mL STEMCELL Technologies 07923 Stemolecule Y27632 Stemgent 04-0012

1 Sendai virus reprogrammed iPSC Lines Line Clone# Sex GAA repeat number in fibroblasts GAA repeat number in iPSCs Earliest passage Karyotype data Karyotype passage 1 CTRL 3348 6 M Control size Control size P5 Yes, normal P11 2 CTRL 7522 5 F Control size Control size P9 Yes, normal P15 3 CTRL 6718 1 M Control size Control size P2 Yes, normal P4 4 CTRL 6718 6 M Control size Control size P2 Yes, normal P4 5 CTRL 6719 1 F Control size Control size

1 FRDA fibroblasts Short alleles <499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele 2 4 M 30 11 597 848 21 M 35 8 622 738 50 M 33 13 353 616 66 M 70 41 110 590 68 F 21 7 570 1200 86 F 30 18 367 780 88 F 50 16 520 520 90 F 29 7 556 556 90B F 33 7 570 570 130 M 56 41

Postdoctoral Position in Neuroimaging A postdoctoral training position is available in the BioMedical Imaging Research Lab (BiMIR) led by Dr. Jiaen Liu in the Advanced Imaging Research Center (AIRC) at UT Southwestern Medical Center to develop advanced high resolution MRI methods for neuroimaging applications. BiMIR has several exciting projects related to novel MRI acquisition and reconstruction, ultrahigh-field MRI (≥7 T), quantitative MRI (T2, T2* and MT) for myelin and iron

DNA Purifi cation Protocol for 4 ml Saliva Samples Cell Lysis 1. Incubate Oragene•DNA/Saliva samples at 50ºC in a water incubator for a minimum of 1 hour or in an air incubator for a minimum of 2 hours. 2. Transfer 4 ml lysate sample (2 ml saliva plus 2 ml Oragene•DNA-preserving solution) to a 15 or 50 ml centrifuge tube. 3. Add 1 ml Cell Lysis Solution and 25 μl Gentra RNase A Solution (4 mg/ml). Vortex on high speed for 10 seconds to mix sample and incubate 10 minutes at room

1 Napierala Lab protocol for human skin fibroblast culture (May 2022) Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Fibroblast Medium recipe: DMEM, high glucose 15% fetal bovine serum (FBS

Postdoctoral Training in Signaling and Gene Regulation using Proteomics and Mass Spectroscopy Dr. W. Lee Kraus is currently seeking applicants with a Ph.D. degree and significant experience in mass spectroscopy for postdoctoral training in the Laboratory of Signaling and Gene Regulation within the Cecil H. and Ida Green Center for Reproductive Biology Sciences. The research in the Kraus lab covers a broad array of topics, including signaling, gene regulation, and genome function

Structural Biology and Biochemistry of Gene Regulation A joint postdoctoral training position is available in the laboratories of Dr. W. Lee Kraus and Dr. Xin Liu in the Cecil H. and Ida Green Center for Reproductive Biology Sciences at UT Southwestern Medical Center to study signaling and gene regulation and molecular biophysics of gene regulation. We are seeking a trainee interested in studying gene regulatory complexes, who will initiate and execute research projects

Postdoctoral Training in Gene Regulation Molecular Mechanisms, Computational Genomics, Mouse Genetic Models, RNA Biology and Noncoding RNAs, Protein Structure, Translational/Clinical A postdoctoral training position is available in the laboratory of Dr. W. Lee Kraus, in the Cecil H. and Ida Green Center for Reproductive Biology Sciences at UT Southwestern Medical Center to study signaling and gene regulation. The Kraus Lab has several exciting projects related to a broad array of

Postdoctoral Training in Bioinformatics and Computational Biology Studies on the Genomics of Gene Regulation A postdoctoral training position is available in the laboratory of Dr. W. Lee Kraus, in the Cecil H. and Ida Green Center for Reproductive Biology Sciences at UT Southwestern Medical Center to study gene regulation on a global scale. The Kraus lab has many exciting, ongoing projects related to the genomics of signal-regulated gene expression in various biological models