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Skip to main content Meet the Team Kexin Yu, Ph.D. Principal Investigator Kexin Yu, Ph.D., LMSW, is a Social Work Scientist and Assistant Professor in the Department of Neurology at UT Southwestern. Her research focuses on reducing loneliness and enhancing cognitive health in older adults through behavioral health interventions and community-based partnerships. Dr. Yu develops and tests programs that promote connection – both in-person and through technology – for individuals with mild cognitive

Towards a molecular pathway for myoblast fusion in Drosophila Elizabeth H. Chen1,2 and Eric N. Olson1 1Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390, USA 2Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA Intercellular fusion among myoblasts is required for the generation of multinucleated muscle fibers during

GE53CH02_Chen ARjats.cls June 17, 2019 14:52 Annual Review of Genetics DrosophilaMyoblast Fusion: Invasion and Resistance for the Ultimate Union Donghoon M. Lee1 and Elizabeth H. Chen1,2 1Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA; email: Elizabeth.Chen@UTSouthwestern.edu 2Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA Annu. Rev. Genet. 2019. 53:2.1–2.25 The Annual Review

Differential splicing and glycosylation of Apoer2 alters synaptic plasticity and fear learning Catherine R. Wasser1,2,*,>†, Irene Masiulis2,†, Murat S. Durakoglugil1,2, Courtney Lane- Donovan1,2, Xunde Xian1,2, Uwe Beffert2, Anandita Agarwala2, Robert E. Hammer3, and Joachim Herz1,2,4,5,* 1Center for Translational Neurodegeneration Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA 2Department of Molecular Genetics, University of Texas Southwestern Medical

1 Sendai virus reprogrammed iPSC Lines Line Clone# Sex GAA repeat number in fibroblasts GAA repeat number in iPSCs Earliest passage Karyotype data Karyotype passage 1 CTRL 3348 6 M Control size Control size P5 Yes, normal P11 2 CTRL 7522 5 F Control size Control size P9 Yes, normal P15 3 CTRL 6718 1 M Control size Control size P2 Yes, normal P4 4 CTRL 6718 6 M Control size Control size P2 Yes, normal P4 5 CTRL 6719 1 F Control size Control size

DNA Purifi cation Protocol for 4 ml Saliva Samples Cell Lysis 1. Incubate Oragene•DNA/Saliva samples at 50ºC in a water incubator for a minimum of 1 hour or in an air incubator for a minimum of 2 hours. 2. Transfer 4 ml lysate sample (2 ml saliva plus 2 ml Oragene•DNA-preserving solution) to a 15 or 50 ml centrifuge tube. 3. Add 1 ml Cell Lysis Solution and 25 μl Gentra RNase A Solution (4 mg/ml). Vortex on high speed for 10 seconds to mix sample and incubate 10 minutes at room

Requirement of the fusogenic micropeptide myomixer for muscle formation in zebrafish Jun Shia,b,1, Pengpeng Bia,b,c,1, Jimin Peid, Hui Lia,b,c, Nick V. Grishind,e, Rhonda Bassel-Dubya,b,c, Elizabeth H. Chena,b,2, and Eric N. Olsona,b,c,2 aDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390; bHamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390; cSenator Paul D. Wellstone Muscular

CELL SCIENCE AT A GLANCE The fusogenic synapse at a glance Ji Hoon Kim1 and Elizabeth H. Chen2,3,* ABSTRACT Cell–cell fusion is a fundamental process underlying fertilization, development, regeneration and physiology of metazoans. It is a multi-step process involving cell recognition and adhesion, actin cytoskeletal rearrangements, fusogen engagement, lipid mixing and fusion pore formation, ultimately resulting in the integration of two fusion partners. Here, we focus on the asymmetric actin

Chen Lab Journal Club The Last Friday of every month, 4:20PM at NA6 small conference room. Date 09.15. Mingjian 10.15. Haocheng 11.15. Seoyun 01.16. Peiqing 02.16. Hui Y. 03.16. Conggang 04.16. Youtong 05.16. Daxing 06.16. Philip 07.16. Tuo 08.16. Hua

1 Napierala Lab protocol for human skin fibroblast culture (May 2022) Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Fibroblast Medium recipe: DMEM, high glucose 15% fetal bovine serum (FBS