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Chen Lab Journal Club The Last Friday of every month, 4:20PM at NA6 small conference room. Date 09.15. Mingjian 10.15. Haocheng 11.15. Seoyun 01.16. Peiqing 02.16. Hui Y. 03.16. Conggang 04.16. Youtong 05.16. Daxing 06.16. Philip 07.16. Tuo 08.16. Hua

We focus on the discovery of targeted therapies for major drivers of cancer using protein chemistry, enzymology, structural biology, informatics and cell biology. Some of our favorite targets are RAS and kinase proteins.

1 Napierala Lab protocol for culturing human iPSCs (May 2022) For details, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSR™1” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number BD MATRIGEL HESC-QUALIFIED Fisher Scientific 08-774-552 mTeSRTM1 Complete Kit STEMCELL Technologies 85850 Cryostor CS10 STEMCELL Technologies 07930 Dispase 1U/mL, 100mL STEMCELL Technologies 07923 Stemolecule Y27632 Stemgent 04-0012

Jiaen Liu, Ph.D. Assistant Professor, Advanced Imaging Research Center and Radiology University of Texas Southwestern Medical Center, Dallas, TX, USA Email: jiaen.liu@utsouthwestern.edu EDUCATION Ph.D. Biomedical Engineering, University of Minnesota, Minneapolis, MN 2015 Minor in Neuroscience, 2011 M.S. Instruments Science and Technology, Tsinghua University, Beijing, China 2007 B.S. Environmental Engineering, Tsinghua University, Beijing, China 2005 RESEARCH EXPERIENCE Assistant Professor

1 FRDA fibroblasts Short alleles <499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele 2 4 M 30 11 597 848 21 M 35 8 622 738 50 M 33 13 353 616 66 M 70 41 110 590 68 F 21 7 570 1200 86 F 30 18 367 780 88 F 50 16 520 520 90 F 29 7 556 556 90B F 33 7 570 570 130 M 56 41

1 P(tBu)2 P(tBu)2 CuBr P Pd P P P PBu3 Pt H Et3P Cl PEt3 Ir S R3P H PR3 H S -S PF6 - Ir S R3P H PR3 H P P Pd PF6 - Pt H Et3P Cl PEt3 Py P P Ir S R3P H PR3 H PF6 - Pt H Et3P Py PEt3 Cl Fe Pt H Et3P Py PEt3 P P Cl- Organometallic Reaction Mechanisms Ligand association/dissociation + toluene insoluble (PBu3)nCuBr soluble + active catalyst for cross coupling = NOTE: No ox. state change Ligand Exchange: Associative - common for 16e- complexes

Postdoctoral Position in Neuroimaging A postdoctoral training position is available in the BioMedical Imaging Research Lab (BiMIR) led by Dr. Jiaen Liu in the Advanced Imaging Research Center (AIRC) at UT Southwestern Medical Center to develop advanced high resolution MRI methods for neuroimaging applications. BiMIR has several exciting projects related to novel MRI acquisition and reconstruction, ultrahigh-field MRI (≥7 T), quantitative MRI (T2, T2* and MT) for myelin and iron

Ready; Catalysis Intro Helpful Texts: Principles and Applications of Organotransition Metal Chemistry, Collman, Hegedus, Norton, Fink The Organometallic Chemistry of the Transition Metals, Crabtree; Prof. M-Christina White’s lecture notes: http://www.courses.fas.harvard.edu/~chem153/ (many of my notes are taken from these, with permission) Advanced Synthesis and Catalysis, Spring 2011 Or: How to make more molecules more complex more quickly with less effort/material/waste

1 Napierala Lab protocol for human skin fibroblast culture (May 2022) Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Fibroblast Medium recipe: DMEM, high glucose 15% fetal bovine serum (FBS

Where does the secreted protein acidic and rich in cysteine (SPARC) protein originate? What leads to its expression? SPARC is secreted from cells. Virtually any cell can produce SPARC. In fact most cells that are adherent in tissue culture produce SPARC at a detectable level. Growth of cells in culture is stressful and SPARC is a protein that is often produced by cells under stress, thus the concept of it as a ‘culture shock’ protein was established. In tissues, cells that are