The Metabolomics Core Facility focuses on targeted metabolomics (quantitation and validation) and untargeted metabolomics (discovery of metabolites).

In Vitro Metabolic Stability Unless a compound is a pro-drug, good metabolic stability is important for achieving therapeutic concentrations for the sufficient amount of time in vivo. The liver is the most important site of drug metabolism in vivo , although other organs (gastrointestinal tract and lung, among others) may play a role. The Core can assess metabolic stability in a variety of different systems, including microsomes, hepatocytes, S9 fractions, and plasma. Microsomes are typically

OMX SR (NL5.120R) 3D structured illumination microscopy (SIM) TIRF SIM 2D PALM/STORM Ring TIRF FRAP, photoactivation Deconvolution Nanopositioning motorized xyz stage Live cell incubation Sign Up Back-to top

Nikon CSU-W1 dual camera (NL5.120R) Spinning disk confocal microscope for fixed or live samples Build for speed, allowing visualization of rapid dynamics in live cells and tissues Can be operated in single camera mode or dual camera mode for true simultaneous 2-channel acquisition. Piezo z-drive with 600 µm travel for fast z-stack acquisition. Fast xy-stage for multi-point acquisition and tiling. Perfect Focus System to keep your sample in focus High acquisition speed insures better cell

Aqueous Solubility Determination Poor aqueous solubility can limit the ability to develop lead compounds as in vivo probes and therapeutics. Aqueous solubility affects absorption, formulation and ability of a compound to be delivered in sufficient concentrations to be active, and even in vitro activity. The Core offers two types of assays: Thermodynamic (or Equilibrium) Solubility Measures solubility of a compound as a saturated solution in equilibrium. Solubility can be dependent on pH and the

Exome Capture Current Protocol Twist Past Protocols SureSelect XT v4 Sample Preparation Guide Instructions for Performing the Nextera Rapid Capture Protocol TruSeq Sample Enrichment Guide Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

mRNA-Seq Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorimeter is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded mRNA Library Prep Kit from Illumina. Poly-A RNA is purified and fragmented before strand specific cDNA synthesis. cDNA are then a-tailed and indexed adapters are

ChIP-Seq Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP beads, then checked on the Agilent 2100 Bioanalyzer to ensure the library is the expected size. Samples are quantified by Qubit before being normalized and pooled, then run on the Illumina HiSeq

The Wellstone Center at UT Southwestern is conducting several research projects into Duchenne muscular dystrophy (DMD).

The Metabolomics Core Facility focuses on targeted metabolomics (quantitation and validation) and untargeted metabolomics (discovery of metabolites).