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The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.

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Contact Us Eugene McDermott Center for Human Growth and Development Nancy B. and Jake L. Hamon Biomedical Research Building North Campus/NA2.138 ( View Campus Map ) 6000 Harry Hines Blvd. Dallas, TX 75235 Phone : 214-648-4913 mdcngs@utsouthwestern.edu the core Mailing Address 5323 Harry Hines Blvd. Dallas, TX 75390-8591 Core Director Ralf Kittler, Ph.D. Director ralf.kittler@utsouthwestern.edu Lab Manager Vanessa Maddox Lab Manager 214-648-5101 vanessa.schmid@utsouthwestern.edu Our Team Jade

Discoveries by Steven McKnight, Ph.D., have revealed the active role of shapeless protein regions known as low-complexity domains in gene regulation.

Mate-Paired Samples are run on a 1.2% agarose gel and their concentrations quantified by fluorometry. Four µg of genomic DNA are processed using the Nextera Mate Pair Sample Preparation Kit from Illumina. Specifically, the Gel-Plus pathway is followed. Samples are first tagmented then subjected to strand displacement, purified with Ampure XP beads and subsequently size selected on .6% megabase agarose gel. After the gel cut, samples are purified using the Zymoclean™ Large Fragment DNA Recovery

Sample Requirements NGS Samples for Library Preparation Submit samples in 1.5 ml Lo-bind microfuge tubes, labeled clearly with order number, submission date, and sample identity. Sample quantification is required prior to submission. NOTE: Concentrations determined by NanoDrop are very unreliable. Please quantify the DNA and RNA concentrations using fluorometry. If you cannot do fluorometry, please send us 2X the minimum amount that NanoDrop determines. Procedure Minimum Quantity RNA Integrity

Whole Genome Samples are run with the Genomic Tape on the Agilent Tapestation 4200 and their concentrations quantified by fluorometery. One microgram genomic DNA is sonicated using Covaris E220 ultrasonicator targeting 400bp. Libraries are prepared with the KAPA Hyper Prep Kit. Samples are end repaired, 3' ends adenylated and barcoded with multiplex adapters. Samples are size selected prior to PCR to remove small fragments. Two cycles of PCR are used to amplify the libraries, which are then

Updates & Accolades UT Southwestern ❘ Discovery@UTSW 2026 ❘ P19 Accolades Drs. Mendell and Pan Elected to National Academy of Medicine Joshua Mendell, M.D., Ph.D., and Duojia Pan, Ph.D., were elected to the National Academy of Medicine, one of the highest honors in health and medicine. Dr. Mendell, Professor of Molecular Biology, has conducted pioneering research on the functions of microRNAs in normal physiology and disease. Dr. Pan, Chair and Professor of Physiology, is recognized for his

When he was just 6 months old, Todd Roberts moved with his family from Houston to Brazil to support his scientist father’s research on malaria and other mosquito-borne diseases. There, he simultaneously learned Brazilian Portuguese and English, becoming the most fluent bilingual speaker in his

For decades, lung cancer has been one of medicine’s toughest opponents. Radiation therapy can slow the disease, but delivering curative doses without harming nearby organs, such as the heart and esophagus has remained out of reach.