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OMX SR (NL5.120R) 3D structured illumination microscopy (SIM) TIRF SIM 2D PALM/STORM Ring TIRF FRAP, photoactivation Deconvolution Nanopositioning motorized xyz stage Live cell incubation Sign Up Back-to top

mRNA-Seq Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorimeter is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded mRNA Library Prep Kit from Illumina. Poly-A RNA is purified and fragmented before strand specific cDNA synthesis. cDNA are then a-tailed and indexed adapters are

The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.

The Metabolomics Core Facility focuses on targeted metabolomics (quantitation and validation) and untargeted metabolomics (discovery of metabolites).

Bioanalysis and Pharmacokinetics Bioanalysis : The Preclinical Pharmacology Core makes use of three combination triple quadrupole/ion trap mass spectrometers from AB Sciex: a 3200 QTRAP® System, a 4000 QTRAP® System, and a QTRAP ® 6500+ System. All three mass spectrometers are coupled to Shimadzu Prominence liquid chromatography instruments with 80 to 100-place autosamplers. Development of an analytical method requires several steps: optimization of an MS/MS method to detect the compound with

Small RNA-Seq Current Protocol NEBNext Small RNA Past Protocol TruSeq Small RNA Sample Preparation Kit Ready to submit samples? Order Online Pricing View Pricing Details Related Core Facilities Discover more information on data analysis services by visiting the Bioinformatics Lab website . Back-to top

Metabolite Profiling If a particular chemical series shows poor metabolic stability, knowledge of the specific metabolites formed can aid chemists in the design of more stable analogues. A comparison of the metabolites formed in different species can also aid in the selection of appropriate species for toxicology studies. The Core has a limited ability to evaluate commonly generated metabolites and to predict the general location of those change based on MS/MS analysis using one of its three

In Vitro Metabolic Stability Unless a compound is a pro-drug, good metabolic stability is important for achieving therapeutic concentrations for the sufficient amount of time in vivo. The liver is the most important site of drug metabolism in vivo , although other organs (gastrointestinal tract and lung, among others) may play a role. The Core can assess metabolic stability in a variety of different systems, including microsomes, hepatocytes, S9 fractions, and plasma. Microsomes are typically

Whole Transcriptome Samples are run on the Agilent Tapestation 4200 to determine level of degradation thus ensuring only high quality RNA is used (RIN Score 8 or higher). The Qubit fluorometer is used to determine the concentration prior to staring library prep. One microgram of total DNAse treated RNA is then prepared with the TruSeq Stranded Total RNA LT Sample Prep Kit from Illumina. Total RNA is depleted of its rRNA and fragmented before strand-specific cDNA synthesis. cDNA are then a-tailed

Aqueous Solubility Determination Poor aqueous solubility can limit the ability to develop lead compounds as in vivo probes and therapeutics. Aqueous solubility affects absorption, formulation and ability of a compound to be delivered in sufficient concentrations to be active, and even in vitro activity. The Core offers two types of assays: Thermodynamic (or Equilibrium) Solubility Measures solubility of a compound as a saturated solution in equilibrium. Solubility can be dependent on pH and the