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APP Mentorship Program for the Office of Advanced Practice Providers, UT Southwestern

Silac Quantitation Introduction Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) is a powerful technique for comparing protein (and peptide) amounts between two or three conditions. Developed in the lab of Matthias Mann it is one of the most widely used labeled quantitation methods in p…

Complex Mixture ID Introduction Modern LC-MS/MS platforms can identify several thousands of proteins from a single sample. We realize that many different types of samples can be submitted for proteomics analsyis, so we accept a wide range of sample formats, including: On-bead (magnetic beads preferre…

Co-IP Experiments Introduction One of the most common types of experiment submitted for analysis in the Proteomics Core is the Co-IP experiment. An antibody against endogenous or tagged protein is used to pull down a protein of interest. The goal of the experiment is to see what comes down with that …

Publications and Expertise The staff of the proteomics core work closely with customers, and are often heavily involved in the write-up process of projects that have made use of mass spectrometry services. In addition to our co-authored papers, our work is frequently acknowledged by our researchers i…

Charging Policy What is our policy for charging users? The Proteomics Core has a simple charging policy: No samples will be prepared or run until the information has been entered into our sample submission site. We charge for every sample run , regardless of whether an expected result is obtained. Th…

Intact Mass What do we use to measure the mass of intact proteins? We do LC/MS analysis of intact proteins using a Q-TOF mass spectrometer with electrospray ionization. This means that samples must be free of detergents. An ideal solvent is 5% acetonitrile in water containing 1-5% formic acid or acet…

General Limitations MS proteomics is a powerful tool for the analysis of protein samples, but there are some limits to the techniques we employ in the Proteomics Core. Knowing the limitations of the services we provide can help to ensure your experiment is a success, and you do not incur costs for un…

Computing and Data Analysis Continuous improvement in mass-spectrometry instrumentation means that proteomics facilities are generating a growing amount of data, and tackling increasingly complex datasets. The rise of quantitative proteomics in particular demands robust data analysis techniques to en…

Learn about the job duties and requirements for a Police Communication Operator with the UT Southwestern Medical Police Department.