Infrastructure & Services

Infrastructure

A custom 4-axis microscope that offers both macroscale and nanoscale imaging of chemically cleared tissues.
The macroscale module provides a lateral and axial resolution of 2.5- and 4.2-microns, respectively, over fields of view from 2.1 mm to 21 mm, ideal for large specimens.
The nanoscale module offers 330 nm resolution across a 340x340 micron field of view.
Fully automated microscope operation and switching between imaging modes – identify biological features of interest with the macroscale module and seamlessly switch to dive deeper with the nanoscale module.
Key upgrades: Addition of a 405 nm laser, enabling operation of the microscope in a cyclic multiplexed format.

Position samples laterally on large travel range stages to interrogate from the top. Particularly useful to analyze cm-sized clinical specimens and facilitates the handling of expanded specimens.
Equipped with an epi-fluorescence module for quick identification of regions of interest.
Resolution of 440 and 330 nm in aqueous and high refractive index media, respectively. Suitable for high-resolution studies of cellular and subcellular features. For expanded tissues, the resolution improves linearly with the expansion coefficient, achieving 22 nm, with molecular specificity, in tissue contexts.

This microscope provides ~700 nm resolution over a 1 mm by 1 mm field of view, ideal for imaging centimeter-sized samples.
Key upgrades: Addition of a numerical aperture 1.15 multi-immersion objective specifically designed for cleared tissue imaging. Increases the photon collection 6-fold and provides ~215 nm lateral resolution.
New image acquisition computer to enable faster, more efficient data collection.

~330 nm isotropic resolution over a 340x340 micron field of view, enabling evaluation of sub-cellular architectures with unparalleled precision.
Key upgrades: Addition of a back-thinned, high quantum efficiency scientific CMOS camera to enable the detection of low-abundance molecular features, ensuring the capture of detailed structures implicated in cancer cell regulation.

~2-micron isotropic resolution across a 2.6 mm field of view, enabling rapid imaging of large areas.
Accompanied by a Mecademic Meca500 industrial robot for automated sample loading and features a custom-designed 24-sample rotary carousel, which can be expanded to accommodate upwards of 60 samples for high-throughput characterization of tissues, metastatic colonization with single cell sensitivity, and other large-scale biological processes.

Optimized for imaging aqueous specimens, such as spheroids and sectioned tissues with ~400 nm lateral and ~580 nm axial resolution.
Captures of volumetric images at speeds of up to 800 planes per second, making it ideal for high-throughput and cyclic immunofluorescence imaging applications.
Equipped with a water-dipping objective enabling the study of complex 3D cellular structures in their native environments.
The single-objective design ensures sample sterility, a critical requirement for live-cell imaging and longitudinal studies, while also supporting multi-well plate formats, rendering the system a powerful tool for targeted pharmacological and genetic screens, and for generating high-resolution insights into cell-cell interactions, drug responses, and dynamic changes within tumor microenvironments.
Key upgrades: Addition of a laser launch for increased spectral multiplexing during cyclic immunofluorescence workflows. Integration of a fluidic exchange system to automate antibody delivery and sample washing, which will significantly increase efficiency, reproducibility, and throughput for large-scale imaging experiments.