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1 Napierala Lab protocol for human skin fibroblast culture (May 2022) Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Fibroblast Medium recipe: DMEM, high glucose 15% fetal bovine serum (FBS

1 Napierala Lab protocol for culturing human iPSCs (May 2022) For details, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSR™1” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number BD MATRIGEL HESC-QUALIFIED Fisher Scientific 08-774-552 mTeSRTM1 Complete Kit STEMCELL Technologies 85850 Cryostor CS10 STEMCELL Technologies 07930 Dispase 1U/mL, 100mL STEMCELL Technologies 07923 Stemolecule Y27632 Stemgent 04-0012

1 FRDA fibroblasts Short alleles <499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele 2 4 M 30 11 597 848 21 M 35 8 622 738 50 M 33 13 353 616 66 M 70 41 110 590 68 F 21 7 570 1200 86 F 30 18 367 780 88 F 50 16 520 520 90 F 29 7 556 556 90B F 33 7 570 570 130 M 56 41

1 updated May 2025 Sendai virus reprogrammed iPSC Lines * Clone used for CRISPR/Cas9 editing of GAA repeats Line Clone # Sex GAA repeat number in fibroblasts GAA repeat number in iPSCs Karyotype data Karyotype passage CTRL 3348 6 M Control size Control size Yes, normal p11 CTRL 7522 5 F Control size Control size Yes, normal p15 CTRL 7522 12 F Control size Control size Yes, normal p13 CTRL 6718 1 M Control size Control size Yes, normal p4 CTRL 6718 6 M

1Scientific RePorTS | (2018) 8:4501 | DOI:10.1038/s41598-018-22819-3 www.nature.com/scientificreports Loss of the adaptor protein ShcA in endothelial cells protects against monocyte macrophage adhesion, LDL-oxydation, and atherosclerotic lesion formation Antoine Abou-Jaoude1, Lise Badiqué1, Mohamed Mlih1, Sara Awan1, Sunning Guo1, Alexandre Lemle1, Clauda Abboud1, Sophie Foppolo1, Lionel Host1, Jérôme Terrand1, Hélène Justiniano1, Joachim Herz2, Rachel L. Matz1 & Philippe Boucher   1

Loss of Reelin protects against atherosclerosis by reducing leukocyte-endothelial adhesion and lesion macrophage accumulation Yinyuan Ding1,2,3,*, Linzhang Huang4,*, Xunde Xian1,2,*, Ivan S. Yuhanna4, Catherine R. Wasser1,2, Michael Frotscher5, Chieko Mineo4, Philip W. Shaul4,†, and Joachim Herz1,2,6,7,8,† 1Department of Molecular Genetics, UT Southwestern Medical Center, Dallas, TX 75390, USA. 2Center for Translational Neurodegeneration Research, UT Southwestern Medical Center, Dallas

1 updated May 2025 Napierala Lab protocol for culturing human iPSCs For additional details and recommendations, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSRTM Plus” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number Corning™ Matrigel™ hESC-Qualified Matrix Fisher Scientific 08-774-552 mTeSRTM Plus Complete Kit STEMCELL Technologies 100-0276 CryoStor® CS10 STEMCELL Technologies 07930 Dispase 1U/mL, 100mL

Differential splicing and glycosylation of Apoer2 alters synaptic plasticity and fear learning Catherine R. Wasser1,2,*,>†, Irene Masiulis2,†, Murat S. Durakoglugil1,2, Courtney Lane- Donovan1,2, Xunde Xian1,2, Uwe Beffert2, Anandita Agarwala2, Robert E. Hammer3, and Joachim Herz1,2,4,5,* 1Center for Translational Neurodegeneration Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA 2Department of Molecular Genetics, University of Texas Southwestern Medical

Postdoctoral Training in Signaling and Gene Regulation using Proteomics and Mass Spectroscopy Dr. W. Lee Kraus is currently seeking applicants with a Ph.D. degree and significant experience in mass spectroscopy for postdoctoral training in the Laboratory of Signaling and Gene Regulation within the Cecil H. and Ida Green Center for Reproductive Biology Sciences. The research in the Kraus lab covers a broad array of topics, including signaling, gene regulation, and genome function

Postdoctoral Training in Gene Regulation Molecular Mechanisms, Computational Genomics, Mouse Genetic Models, RNA Biology and Noncoding RNAs, Protein Structure, Translational/Clinical A postdoctoral training position is available in the laboratory of Dr. W. Lee Kraus, in the Cecil H. and Ida Green Center for Reproductive Biology Sciences at UT Southwestern Medical Center to study signaling and gene regulation. The Kraus Lab has several exciting projects related to a broad array of