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JCB: Article The Rockefeller University Press   $30.00 J. Cell Biol. Vol. 199 No. 1  169–185 www.jcb.org/cgi/doi/10.1083/jcb.201204065 JCB 169 R. Duan and P. Jin contributed equally to this paper. Correspondence to Elizabeth H. Chen: echen@jhmi.edu Abbreviations used in this paper: AID, auto-inhibitory domain; DPak, Drosophila p21-activated kinase; Duf, Dumbfounded; Eve, Even skipped; FCM, fusion- competent myoblast; MHC, myosin heavy chain; NPF, nucleation-promoting factor; PAK, p21

User manual for TYGRESS Tomography-Guided 3D Reconstruction of Subcellular Structures (TYGRESS) is a hybrid method, which combines the advantageous features of both single particle cryo-EM and cryo-ET/subtomogram averaging to provide high-resolution structures of macromolecular complexes within their native, cellular environment. In this method, one typical single particle cryo-EM micrograph is acquired (i.e., with an electron dose that is higher than for individual tilt series

Developmental Cell, Vol. 1, 705–715, November, 2001, Copyright 2001 by Cell Press Antisocial, an Intracellular Adaptor Protein, Is Required for Myoblast Fusion in Drosophila founder cells while the remaining twist-expressing cells become fusion competent (for reviews, see Baylies et al., 1998; Frasch, 1999). It is believed that the founder Elizabeth H. Chen and Eric N. Olson1 Department of Molecular Biology University of Texas Southwestern Medical Center at Dallas cells serve as sources of

1 updated May 2025 Napierala Lab protocol for human skin fibroblast culture Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Dimethyl sulfoxide (DMSO) Sigma D8418-100ML Fibroblast Medium recipe

December 26, 2016 Chen Lab Weekly Meeting Schedule Wednesday at 9 am (unless indicated otherwise), NA6.214 1/4/17 Chee-Kwee & Jueqi 1/19/17 (Thursday) Mingjian & Shuiqing (NA8.218) 1/24/17 (Tuesday) Junyao & Seoyun (NA8.218) 2/3/17 (Friday) Youtong & Fenghe 2/8/17 Nicole & Philip 2/15/17 Xiang G & Xiang C 2/24/17 (Friday) Ming & Hanze W 3/1/17 Conggang & Hua 3/8/17 Peiqing & Hui Y 3/15/17 Xiaojun & Junjiao 3/24/17 (Friday

Kidney Practice Practical Questions: 1. Bowman's space 2. Bowman's capsule 3. Parietal epithelial cell 4. Mesangial cell 5. Glomerular capillary 6. Urinary pole 7. Vascular pole 8. Cell that senses filtrate osmolarity 9. Cell that produces renin 10. Nucleus of a proximal convoluted epithelial cell 11. Nucleus of a proximal straight epithelial cell 12. Nucleus of a loop of Henle epithelial cell 13. Nucleus of a distal straight epithelial cell 14. Nucleus of a distal convoluted

GE53CH02_Chen ARjats.cls June 17, 2019 14:52 Annual Review of Genetics DrosophilaMyoblast Fusion: Invasion and Resistance for the Ultimate Union Donghoon M. Lee1 and Elizabeth H. Chen1,2 1Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA; email: Elizabeth.Chen@UTSouthwestern.edu 2Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA Annu. Rev. Genet. 2019. 53:2.1–2.25 The Annual Review

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Didactic Answers 1. Epimysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 2. Perimysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 3. Endomysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 4. Fascicles are A. groupings of

DNA Purifi cation Protocol for 4 ml Saliva Samples Cell Lysis 1. Incubate Oragene•DNA/Saliva samples at 50ºC in a water incubator for a minimum of 1 hour or in an air incubator for a minimum of 2 hours. 2. Transfer 4 ml lysate sample (2 ml saliva plus 2 ml Oragene•DNA-preserving solution) to a 15 or 50 ml centrifuge tube. 3. Add 1 ml Cell Lysis Solution and 25 μl Gentra RNase A Solution (4 mg/ml). Vortex on high speed for 10 seconds to mix sample and incubate 10 minutes at room