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| PERSPECTIVES Sex and the Single Fly: A Perspective on the Career of Bruce S. Baker Deborah J. Andrew,* Elizabeth H. Chen,†,‡,§ Devanand S. Manoli,**,†† Lisa C. Ryner,‡‡ and Michelle N. Arbeitman§§,1 *Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, †Department of Molecular Biology, ‡Department of Cell Biology, §and Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390

Page 1 of 11 DO NOT DISCLOSE The University of Texas Southwestern Medical Center CONSENT TO PARTICIPATE IN RESEARCH YOU WILL BE GIVEN A COPY OF THIS CONSENT FORM TO KEEP Title of Research: Preventing Perinatal Depression (PRE-D): Developing Tools and Interventions - Diagnostic Evaluation Phase Funding Agency/Sponsor: National Institute of Mental Health (NIMH) Study Psychologists: Telephone No. Telephone No. (Regular office hours) (Other times) 1

Invasive Podosomes and Myoblast Fusion Elizabeth H. Chen Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA I. OVERVIEW Myoblast fusion is critical for the development, maintenance, and regeneration of skeletal muscles. Despite the identification of many fusion-related molecules in the past decades, the cellular mechanics of myoblast membrane fusion have just begun to be understood. Recent studies using the fruit fly

Eugene McDermott Center for Human Growth and Development Southwestern Medical School 5323 Harry Hines Blvd. / Dallas, Texas 75390-8591 (214) 648-1695 Fax (214) 648-1666 Email: chao.xing@utsouthwestern.edu Computational Biologist I The laboratories of Dr. Ralph DeBerardinis at Children’s Research Institute (https://cri.utsw.edu/scientists/ralph-deberardinis-laboratory/) and Dr. Chao Xing at McDermott Center for Human Growth and Development (https://labs.utsouthwestern.edu

\ PEGSITACIANINE FOR THE SURGICAL DETECTION OF HEAD AND NECK CANCER PURPOSE INCLUSION DESIGN To investigate whether Pegsitacianine a fluorescent nanoparticle can image head and neck cancer and unknown primary head and neck cancer during surgery or biopsy. 1. Adults ≥ 18 years of age 2. Patients with stage 1-4 Head and Neck Squamous Cell Carcinoma (HNSCC) undergoing surgical excision 1. Phase 2 Clinical trial of Pegsitacianine, which is

O:\PSYCHDEP\PRE-D\Recruitment\Approved IRB Documents_6_17_14\Clinician of Record Agreement_6_19_14.doc Clinician of Record’s Agreement to Exchange Information I understand that my patient _______________________________________________ is seeking to participate in the National Institute of Mental Health (NIMH) sponsored research study, Preventing Perinatal Depression: Developing Tools and Interventions (PRE-D), in the Department of Psychiatry at UT Southwestern. She signed a two-way

Zhongzheng (Brooks) Fu, Ph.D. Last updated: September, 2023 ORCID: 0000-0002-2572-6284 Email: zhongzheng.fu@utsouthwestern.edu Website: zfu-lab.org 5323 Harry Hines Blvd, MC 8855 Dallas, TX 75390 United States Academic Positions 2023 – on Assistant Professor Department of Neurological Surgery UT Southwestern Medical Center Dallas, TX, USA 2023 – on Visiting Scholar Division of the Humanities and Social Sciences California Institute of Technology Pasadena, CA, USA Postdoctoral Trainings

Bu cc al B ru sh Gentra Puregene Handbook 06/201128 Protocol: DNA Purification from a Buccal Brush Using the Gentra Puregene Buccal Cell Kit This protocol is for purification of genomic DNA from 1 buccal brush using the Gentra Puregene Buccal Cell Kit. Things to do before starting � Preheat water baths to 55°C for use in step 3b and 65°C for use in steps 3a and 17 of the procedure. � Optional: Preheat water bath to 37°C for use in step 5 of the procedure. Procedure 1. To collect

Single Cell Transcriptomics – a simple walkthrough Adwait Sathe, PhD McDermott Center Bioinformatics Lab adwait.sathe@utsouthwestern.edu MCDERMOTT CENTER BIOINFORMATICS LAB 1 mailto:Adwait.sathe@utsouthwestern.edu RNASeq RNASeq 2 MCDERMOTT CENTER BIOINFORMATICS LAB https://www.10xgenomics.com/solutions/single-cell/ Single cell RNA Sequencing Workflow Dissociation of solid tissue Isolate single cells DROP-Seq, Smart-Seq etc. RNA cDNA Amplified RNA Amplified cDNA Sequencing

ARTICLE OPEN Lrp1 in osteoblasts controls osteoclast activity and protects against osteoporosis by limiting PDGF–RANKL signaling Alexander Bartelt1,2,8, Friederike Behler-Janbeck1,2, F. Timo Beil1,3, Till Koehne3,4, Brigitte Müller1,2, Tobias Schmidt1,2, Markus Heine2,5, Laura Ochs1,2, Tayfun Yilmaz3, Martin Dietrich6, Jan P. Tuckermann7, Michael Amling3, Joachim Herz6, Thorsten Schinke3, Joerg Heeren2 and Andreas Niemeier1,2 Skeletal health relies on architectural integrity and sufficient