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Immunohistochemistry: CORT - UT Southwestern, Dallas, TX

https://www.utsouthwestern.edu/research/cort/research-projects-support/protocols/immunohistochemistry.html

Protocol for immunohistochemistry.

Set Up, Manage & Close Out Awards: Sponsored Programs - For Employees - UT Southwestern, Dallas, Texas

https://www.utsouthwestern.edu/research/spa/process/clinical-research/awards.html

Set Up Award provides practical steps to ensure your award is properly setup.

Awards Setup, Management & Closeout: Sponsored Programs - For Employees - UT Southwestern, Dallas, Texas

https://www.utsouthwestern.edu/research/spa/process/basic-science/awards.html

Awards Setup, Management & Closeout

Mechanical Tension Drives Cell Membrane Fusion

https://labs.utsouthwestern.edu/sites/default/files/2022-08/kim-2015.pdf

Article Mechanical Tension Drives Cell Membrane Fusion Graphical Abstract Highlights d Invasive protrusions trigger a mechanosensory response in a cell-fusion partner d Mechanosensory function of MyoII directs its accumulation at the fusogenic synapse d MyoII increases cortical tension and promotes fusion pore formation d Mechanical tension at the fusogenic synapse drives cell membrane fusion Kim et al., 2015, Developmental Cell 32, 561–573 March 9, 2015 ª2015 Elsevier Inc. http

nihms653025.pdf

https://labs.utsouthwestern.edu/sites/default/files/2022-07/nihms653025.pdf

Differential splicing and glycosylation of Apoer2 alters synaptic plasticity and fear learning Catherine R. Wasser1,2,*,>†, Irene Masiulis2,†, Murat S. Durakoglugil1,2, Courtney Lane- Donovan1,2, Xunde Xian1,2, Uwe Beffert2, Anandita Agarwala2, Robert E. Hammer3, and Joachim Herz1,2,4,5,* 1Center for Translational Neurodegeneration Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA 2Department of Molecular Genetics, University of Texas Southwestern Medical

shilagardi-2013.pdf

https://labs.utsouthwestern.edu/sites/default/files/2022-08/shilagardi-2013.pdf

Actin-propelled Invasive Membrane Protrusions Promote Fusogenic Protein Engagement During Cell-Cell Fusion Khurts Shilagardi1, Shuo Li1, Fengbao Luo1, Faiz Marikar1, Rui Duan1, Peng Jin1, Ji Hoon Kim1, Katherine Murnen1, and Elizabeth H. Chen1,* 1Dept. of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA Abstract Cell-cell fusion is critical for the conception, development and physiology of multicellular organisms. Although cellular

Loss of the adaptor protein ShcA in endothelial cells protects against monocyte macrophage adhesion, LDL-oxydation, and atherosclerotic lesion formation

https://labs.utsouthwestern.edu/sites/default/files/2022-07/41598_2018_Article_22819.pdf

1Scientific RePorTS | (2018) 8:4501 | DOI:10.1038/s41598-018-22819-3 www.nature.com/scientificreports Loss of the adaptor protein ShcA in endothelial cells protects against monocyte macrophage adhesion, LDL-oxydation, and atherosclerotic lesion formation Antoine Abou-Jaoude1, Lise Badiqué1, Mohamed Mlih1, Sara Awan1, Sunning Guo1, Alexandre Lemle1, Clauda Abboud1, Sophie Foppolo1, Lionel Host1, Jérôme Terrand1, Hélène Justiniano1, Joachim Herz2, Rachel L. Matz1 & Philippe Boucher   1

untitled

https://labs.utsouthwestern.edu/sites/default/files/2022-06/Qiagen%20Protocol%20for%20DNA%20Purification%20from%20Buccal%20Sample.pdf

Bu cc al B ru sh Gentra Puregene Handbook 06/201128 Protocol: DNA Purification from a Buccal Brush Using the Gentra Puregene Buccal Cell Kit This protocol is for purification of genomic DNA from 1 buccal brush using the Gentra Puregene Buccal Cell Kit. Things to do before starting � Preheat water baths to 55°C for use in step 3b and 65°C for use in steps 3a and 17 of the procedure. � Optional: Preheat water bath to 37°C for use in step 5 of the procedure. Procedure 1. To collect

Spectrin is a mechanoresponsive protein shaping fusogenic synapse architecture during myoblast fusion

https://labs.utsouthwestern.edu/sites/default/files/2022-08/spectrin-paper-2018.pdf

Articles https://doi.org/10.1038/s41556-018-0106-3 1Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. 2Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. 3Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX, USA. 4Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA. 5Departments of Biology and of Biochemistry and Molecular Biology

3DPM Quantification, Pediatric Cardiology, UT Southwestern, Dallas, Texas

https://labs.utsouthwestern.edu/sites/default/files/2022-06/tandon-3dpm-quantification.pdf

Accurate 3D Prints for RVOT Interventions: A Quantitative Study Animesh Tandon, MD, MS1-4; Rami Hallac, PhD4; Nicholas Byrne, MSc5, 6; Maria de las Nieves Velasco Forte, MD5; Gerald F. Greil, MD, PhD1-5; Tarique Hussain, MD, PhD1-5 1Departments of Pediatrics, 2Radiology, and 3Biomedical Engineering, University of Texas Southwestern Medical Center, Dallas, Texas 4Children’s Medical Center Dallas, Dallas, Texas 5Division of Imaging Sciences and Biomedical Engineering, King’s College London