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Meet the people of the Center for Organogenesis, Regeneration and Trauma at UT Southwestern.

The Protein Chemistry Technology Core at UT Southwestern offers peptide synthesis and Edman protein sequencing to the academic research community.

Whole Genome Samples are run with the Genomic Tape on the Agilent Tapestation 4200 and their concentrations quantified by fluorometery. One microgram genomic DNA is sonicated using Covaris E220 ultrasonicator targeting 400bp. Libraries are prepared with the KAPA Hyper Prep Kit. Samples are end repaired, 3' ends adenylated and barcoded with multiplex adapters. Samples are size selected prior to PCR to remove small fragments. Two cycles of PCR are used to amplify the libraries, which are then

Mate-Paired Samples are run on a 1.2% agarose gel and their concentrations quantified by fluorometry. Four µg of genomic DNA are processed using the Nextera Mate Pair Sample Preparation Kit from Illumina. Specifically, the Gel-Plus pathway is followed. Samples are first tagmented then subjected to strand displacement, purified with Ampure XP beads and subsequently size selected on .6% megabase agarose gel. After the gel cut, samples are purified using the Zymoclean™ Large Fragment DNA Recovery

Price List Analyses are charged per sample submitted. Any gel band or slice must be 1 cm or less of the gel, and must be diced into ~1mm cubes. Gel slices that are not diced or that are minced too fine may be sent back. We charge for all samples , whether or not a result is obtained. The only exception is where failure is due to processing or instrumentation problems in the core. Please see our charging policy for details . Billing / Charging Samples from UTSW customers will require a COA for

Client Microscopy Facilities The Histo Pathology Core has microscopy facilities for clients to review and document completed histology slides, as well as to document wet tissues and cell culture preps that augment their histopathology. Self-Service Microscopy by Reservation These instruments, located in the Core's Tissue Handling Lab (NB11.204), are offered on a self-service, reservation basis: Leica DM2000 Upright Compound Research Photomicroscope Bright-Field, Dark-Field, Epifluorescence

Older Publications for the Preclinical Pharmacology Core at UT Southwestern

Routine Histology The Histo Pathology Core offers routine histology services for workup of specimens as paraffin or frozen. Routine histology services offered include: Paraffin Processing and Embedding: Automated, Manual, Chromagen Cryoembedding: TFM, OCT, or Gum Tragacanth Paraffin Sectioning: H&Es and/or unstained slides cut to needed anatomy Cryosectioning: H&Es and/or unstained slides cut to needed anatomy Additional Levels: Ribbon, Interval, Additional Block, Serials Separate Slide Special

Establish a Relationship details how to identify a sponsored program that might be a good fit and cultivate a sponsor relationship.