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Dr. Levine, a Professor of Internal Medicine and Microbiology and holder of the Charles Sprague Distinguished Chair in Biomedical Science at UT Southwestern, was known for her incisive intellect, demand for scientific rigor, and commitment to mentoring. Her leadership in both clinical medicine and

The Electron Microscopy Core Facility (EMCF) offers TEM of cells & tissues, negative staining, whole-mount SEM, correlative LM and EM, and Immunogold labeling.

Specialized Techniques The Histo Pathology Core offers services that are irregular offshoots of histology, and usually only performed in individual academic or pharmaceutical research lab settings. Services, such as Laser Microdissection and others listed on this page, are technically demanding and somewhat slow throughput, but are of great interest to select clients. The Core created this separate page as a catch-all for these service offerings that are not easily characterized within any of

Special Stains The Histo Pathology Core offers a wide menu of special stains for improving visualization of normal and disease state chemical moieties. Special Stains that the Core performs on a routine schedule include Oil Red-O stain for neutral lipids, Masson's Trichrome stain for collagen/fibrosis, Periodic-Acid Schiff for sugars and basement membrane, as well as Hart's variation of the Verhoeff Van Gieson's technique for elastin. Optimum staining for some substances requires a specific

Anatomic Pathology The Histo Pathology Core offers anatomic pathology services on an as needed basis to train clients as well as facilitate morphologic and histologic endpoints in large terminal studies with mice. The Core provides expert training in the following procedures with mice: Transcardial Whole Body Perfusion All Organs Survey Dissection Organ Specific Dissection Organ Specific Grossing Embryo Collection and Staging (E8 - E15) The Core maintains animal use protocols covering the

Aqueous Solubility Determination Poor aqueous solubility can limit the ability to develop lead compounds as in vivo probes and therapeutics. Aqueous solubility affects absorption, formulation and ability of a compound to be delivered in sufficient concentrations to be active, and even in vitro activity. The Core offers two types of assays: Thermodynamic (or Equilibrium) Solubility Measures solubility of a compound as a saturated solution in equilibrium. Solubility can be dependent on pH and the

Nikon CSU-W1 SoRa (NL5.120R) Spinning disk confocal microscope for fixed or live samples Build for speed, allowing visualization of rapid dynamics in live cells and tissues SoRa module for resolution down to 120 nm. Piezo z-drive for fast z-stack acquisition. Fast xy-stage for multi-point acquisition and tiling. Perfect Focus System to keep your sample in focus High acquisition speed ensures better cell viability and reduced photobleaching compared to point-scanning LSM. 405, 445, 488, 514, 561

In Vitro Metabolic Stability Unless a compound is a pro-drug, good metabolic stability is important for achieving therapeutic concentrations for the sufficient amount of time in vivo. The liver is the most important site of drug metabolism in vivo , although other organs (gastrointestinal tract and lung, among others) may play a role. The Core can assess metabolic stability in a variety of different systems, including microsomes, hepatocytes, S9 fractions, and plasma. Microsomes are typically

Metabolite Profiling If a particular chemical series shows poor metabolic stability, knowledge of the specific metabolites formed can aid chemists in the design of more stable analogues. A comparison of the metabolites formed in different species can also aid in the selection of appropriate species for toxicology studies. The Core has a limited ability to evaluate commonly generated metabolites and to predict the general location of those change based on MS/MS analysis using one of its three

The PCIRCF at UT Southwestern uses iLab, an online platform that streamlines equipment reservations, consultations, and billing for core services.