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Page 1 of 11 DO NOT DISCLOSE The University of Texas Southwestern Medical Center CONSENT TO PARTICIPATE IN RESEARCH YOU WILL BE GIVEN A COPY OF THIS CONSENT FORM TO KEEP Title of Research: Preventing Perinatal Depression (PRE-D): Developing Tools and Interventions - Diagnostic Evaluation Phase Funding Agency/Sponsor: National Institute of Mental Health (NIMH) Study Psychologists: Telephone No. Telephone No. (Regular office hours) (Other times) 1

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1 Joseph Martin Ready Department of Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd. Dallas, TX 75390-9038 (214) 648-0313 joseph.ready@utsouthwestern.edu Employment 2012 – Professor, UT Southwestern 2009 – Director, Medicinal Chemistry Laboratory, UT Southwestern 2007 – 2012 Associate Professor, UT Southwestern 2007 – Chair, chemistry training track, UT Southwestern 2003 – 2007 Assistant Professor, UT Southwestern and

Vinogradov Lab alumni

Bu cc al B ru sh Gentra Puregene Handbook 06/201128 Protocol: DNA Purification from a Buccal Brush Using the Gentra Puregene Buccal Cell Kit This protocol is for purification of genomic DNA from 1 buccal brush using the Gentra Puregene Buccal Cell Kit. Things to do before starting � Preheat water baths to 55°C for use in step 3b and 65°C for use in steps 3a and 17 of the procedure. � Optional: Preheat water bath to 37°C for use in step 5 of the procedure. Procedure 1. To collect

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1 updated May 2025 Napierala Lab protocol for human skin fibroblast culture Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-050 0.25% Trypsin-EDTA, phenol red Gibco/Life Technologies 25200-056 DPBS (1X) Gibco/Life Technologies 14190-144 Dimethyl sulfoxide (DMSO) Sigma D8418-100ML Fibroblast Medium recipe

Chen (Zhijian "James") Lab' Resources

1 updated May 2025 FRDA fibroblasts Short alleles < 499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual nd - not determined Cell Line Sex Sampling Age Age of Onset GAA repeats Allele 1 Allele 2 4 M 30 11 597 848 21 M 35 8 622 738 50 M 33 13 353 616 66 M 70 41 110 590 68 F 21 7 570 1200 86 F 30 18 367 780 88 F 50 16 520 520 90 F 29 7 556 556

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