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Complex Mixture ID

https://www.utsouthwestern.edu/research/core-facilities/proteomics/services/complex-mixture-id.html

Complex Mixture ID Introduction Modern LC-MS/MS platforms can identify several thousands of proteins from a single sample. We realize that many different types of samples can be submitted for proteomics analsyis, so we accept a wide range of sample formats, including: On-bead (magnetic beads preferre…

Establish Relationship: Sponsored Programs - For Employees - UT Southwestern, Dallas, Texas

https://www.utsouthwestern.edu/research/spa/process/clinical-research/establish-relationship.html

Establish a Relationship details how to identify a sponsored program that might be a good fit and cultivate a sponsor relationship.

Price List

https://www.utsouthwestern.edu/research/core-facilities/proteomics/pricing/price-list.html

Price List Analyses are charged per sample submitted. Any gel band or slice must be 1 cm or less of the gel, and must be diced into ~1mm cubes. Gel slices that are not diced or that are minced too fine may be sent back. We charge for all samples , whether or not a result is obtained. The only excepti…

Charging Policy

https://www.utsouthwestern.edu/research/core-facilities/proteomics/pricing/charging-policy.html

Charging Policy What is our policy for charging users? The Proteomics Core has a simple charging policy: No samples will be prepared or run until the information has been entered into our sample submission site. We charge for every sample run , regardless of whether an expected result is obtained. Th…

Cutting Gels

https://www.utsouthwestern.edu/research/core-facilities/proteomics/services/cutting-gels.html

Cutting Gels Introduction Many proteomics samples are well suited for SDS-PAGE, including protein identification and PTM identification. We recognize that a gel approach may not be compatible with all samples, so please contact us if you have concerns about running your sample into a gel. We require …

Co-IP Experiments

https://www.utsouthwestern.edu/research/core-facilities/proteomics/services/co-ip-experiments.html

Co-IP Experiments Introduction One of the most common types of experiment submitted for analysis in the Proteomics Core is the Co-IP experiment. An antibody against endogenous or tagged protein is used to pull down a protein of interest. The goal of the experiment is to see what comes down with that …

TMT Quantitation

https://www.utsouthwestern.edu/research/core-facilities/proteomics/services/tmt-quantitation.html

TMT Quantitation Introduction Tandem Mass Tag (TMT) technology is a powerful technique for comparing protein (and peptide) amounts between six to eighteen conditions. This technique involves labeling samples after digestion, meaning this technique can be used on in vivo samples, unlike SILAC. TMT or …

Intact Mass

https://www.utsouthwestern.edu/research/core-facilities/proteomics/services/intact-mass.html

Intact Mass What do we use to measure the mass of intact proteins? We do LC/MS analysis of intact proteins using a Q-TOF mass spectrometer with electrospray ionization. This means that samples must be free of detergents. An ideal solvent is 5% acetonitrile in water containing 1-5% formic acid or acet…

General Limitations

https://www.utsouthwestern.edu/research/core-facilities/proteomics/services/general-limitations.html

General Limitations MS proteomics is a powerful tool for the analysis of protein samples, but there are some limits to the techniques we employ in the Proteomics Core. Knowing the limitations of the services we provide can help to ensure your experiment is a success, and you do not incur costs for un…

ChIP-Seq - Next Generation Sequencing Core

https://www.utsouthwestern.edu/research/core-facilities/next-generation-sequencing/ngs-applications/chip-seq.html

ChIP-Seq Libraries from 5–10 ng ChIP DNA are prepared using KAPA HTP Library Preparation Kit. The ChIP DNA is quantified on the Qubit® 2.0 Fluorometer from Invitrogen Samples are end repaired, 3' ends adenylated and barcoded with mµltiplex adapters. PCR amplified libraries are purified with Ampure XP…