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Muscle Practice Didactic Answers
https://labs.utsouthwestern.edu/sites/default/files/2022-08/muscle-answers.pdf
Didactic Answers 1. Epimysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skeletal muscles 2. Perimysium surrounds A. skeletal muscle fibers B. cardiomyocytes C. smooth muscle cells D. skeletal muscle fascicles E. skele…
Microsoft Word - FACLR_Human_iPSCs_SOP_2025.docx
https://labs.utsouthwestern.edu/sites/default/files/2025-05/FACLR_Human_iPSCs_SOP_2025.pdf
1 updated May 2025 Napierala Lab protocol for culturing human iPSCs For additional details and recommendations, please refer to “Maintenance of Human Pluripotent Stem Cells in mTeSRTM Plus” Technical Manual (STEMCELL Technologies). Necessary Reagents Reagent Company Catalog Number …
Microsoft Word - FACLR_iPSC_05_2025_JSN.docx
https://labs.utsouthwestern.edu/sites/default/files/2025-05/FACLR_iPSC_lines_2025_0.pdf
1 updated May 2025 Sendai virus reprogrammed iPSC Lines * Clone used for CRISPR/Cas9 editing of GAA repeats Line Clone # Sex GAA repeat number in fibroblasts GAA repeat number in iPSCs Karyotype data Karyotype passage CTRL 3348 6 M Control size Control size Yes, normal p…
Microsoft Word - FACLR_Human_fibroblast_SOP_2025.docx
https://labs.utsouthwestern.edu/sites/default/files/2025-05/FACLR_Human_fibroblast_SOP_2025_0.pdf
1 updated May 2025 Napierala Lab protocol for human skin fibroblast culture Necessary Reagents Reagent Company Catalog Number DMEM, high glucose Gibco/Life Technologies 11965-092 Fetal bovine serum Hyclone SH30910.03 MEM Non-essential amino acids (NEAA) Gibco/Life Technologies 11140-05…
Microsoft Word - FACLR_fibroblasts_2025.docx
https://labs.utsouthwestern.edu/sites/default/files/2025-05/FACLR_fibroblast_lines_2025_1.pdf
1 updated May 2025 FRDA fibroblasts Short alleles < 499 repeats Medium alleles 500 – 999 repeats Long alleles >1000 repeats B - indicates line established from a second, independent skin biopsy of the same individual nd - not determined Cell Line Sex Sampling Age Age of …
https://labs.utsouthwestern.edu/sites/default/files/2022-07/1240.1-20201218131541-covered-e0fd13ba177f913fd3156f593ead4cfd.pdf
Inducible inactivation of hepatic LRP gene by cre-mediated recombination confirms role of LRP in clearance of chylomicron remnants. A Rohlmann, … , R E Hammer, J Herz J Clin Invest. 1998;101(3):689-695. https://doi.org/10.1172/JCI1240. The multifunctional low density lipoprotein (LDL) receptor-rel…
https://labs.utsouthwestern.edu/sites/default/files/2022-08/Sens-2010.pdf
JCB: Article The Rockefeller University Press $30.00 J. Cell Biol. Vol. 191 No. 5 1013–1027 www.jcb.org/cgi/doi/10.1083/jcb.201006006 JCB 1013 K.L. Sens and S. Zhang contributed equally to this paper. P. Jin and R. Duan contributed equally to this paper. Correspondence to Elizabeth H. Chen: eche…
https://labs.utsouthwestern.edu/sites/default/files/2022-08/jin-2011.pdf
Competition between Blown Fuse and WASP for WIP binding regulates the dynamics of WASP-dependent actin polymerization in vivo Peng Jin1,*, Rui Duan1,*, Fengbao Luo1, Guofeng Zhang2, Sabrina N. Hong1, and Elizabeth H. Chen1,3 1 Dept. of Molecular Biology and Genetics, Johns Hopkins University School…
Drosophila Myoblast Fusion: Invasion and Resistance for the Ultimate Union
https://labs.utsouthwestern.edu/sites/default/files/2022-08/lee-chen%202019.pdf
GE53CH02_Chen ARjats.cls June 17, 2019 14:52 Annual Review of Genetics DrosophilaMyoblast Fusion: Invasion and Resistance for the Ultimate Union Donghoon M. Lee1 and Elizabeth H. Chen1,2 1Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA; em…
Spectrin is a mechanoresponsive protein shaping fusogenic synapse architecture during myoblast fusion
https://labs.utsouthwestern.edu/sites/default/files/2022-08/spectrin-paper-2018.pdf
Articles https://doi.org/10.1038/s41556-018-0106-3 1Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. 2Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. 3Department of Molecular Biology, UT South…