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10 to 70% methanol in 50 mM KH2PO4 over 25 min, 10 ml/min, monitor at 380 nm). Next, the HPLC- purified mixture was desalted on the same column (methanol was removed on a rotary evaporator, and the sample loaded in H2O and eluted with 90% methanol) and lyophilized, yielding the purified Nvoc-aa-S-CoA…

Surface hydrolysis of poly(glycolic acid) meshes increases the seeding density of vascular smooth muscle cells Jinming Gao,1,2 Laura Niklason,1,2 Robert Langer1 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 2Department of Anesthesia, Mass…

COMMUNICATIONS Surface Modification of Polyanhydride Microspheres JINMING GAO†,‡, LAURA NIKLASON†,‡, XIAO-MEI ZHAO§, AND ROBERT LANGER*,† Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, Department of Anesthesia, Massachusetts General Hospi…

An NQO1- and PARP-1-mediated cell death pathway induced in non-small-cell lung cancer cells by �-lapachone Erik A. Bey†‡§, Melissa S. Bentle¶, Kathryn E. Reinicke�, Ying Dong†‡, Chin-Rang Yang‡, Luc Girard††, John D. Minna††, William G. Bornmann‡‡, Jinming Gao‡, and David A. Boothman†‡§ †Departments…

Probing the Energetics of Dissociation of Carbonic Anhydrase-Ligand Complexes in the Gas Phase Jinming Gao,* Qinyuan (Quincey) Wu,# Jeffrey Carbeck,* Q. Paula Lei,# Richard D. Smith,# and George M. Whitesides* *Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusett…

628 graft morphology and function have not been adequately addressed. A systematic understanding of the effects of in vitro parameters will be important if these culture tech- niques are to be translated to human cells for the develop- ment of autologous human vascular grafts that have acceptable lo…

Protein Charge Ladders, Capillary Electrophoresis, and the Role of Electrostatics in Biomolecular Recognition JEFFREY D. CARBECK, IAN J. COLTON, JINMING GAO, AND GEORGE M. WHITESIDES* Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 Received September …

Journal of Controlled Release 83 (2002) 415–425 www.elsevier.com/ locate/ jconrel N oninvasive monitoring of local drug release in a rabbit radiofrequency (RF) ablation model using X-ray computed tomography a b a aAgata Szymanski-Exner , Nicholas T. Stowe , Roee S. Lazebnik , Kyle Salem , a c a,c …

Using Protein Charge Ladders To Estimate the Effective Charges and Molecular Weights of Proteins in Solution Jinming Gao and George M. Whitesides* Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138 This paper describes the use of capi…

Carbonic Anhydrase-Inhibitor Binding: From Solution to the Gas Phase Qinyuan Wu,†,§ Jinming Gao,‡ Diane Joseph-McCarthy,‡ George B. Sigal,‡ James E. Bruce,† George M. Whitesides,*,‡ and Richard D. Smith*,† EnVironmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland,…