Module 1 - Tissue Culture/Monoclonal Antibody Production

The Tissue Culture/Monoclonal Antibody Module provides the following services:

  • Routine maintenance of immortalized and continuous cell lines
  • Maintenance of cryopreserved stocks of cell lines
  • Establishment of primary corneal and retinal cell lines
  • Maintenance of a tumor bank of human uveal melanoma cell lines and acquisition of new uveal melanoma cell lines from collaborators
  • Cultivation of hybridomas
  • Affinity purification and concentration of monoclonal antibodies
  • Routine screening of cell lines and hybridomas for mycoplasma and endotoxin contamination
  • Production of immortalized cell lines using SV40 large T antigen, E6/E7, or telomerase gene transfer techniques
  • Generation of adenovirus vectors and insertion of various plasmids containing cDNA gene constructs (e.g., murine IFN-β cDNA) into adenovirus vectors for gene transfer experiments

This Module has four fundamental goals:

  • To free the individual investigator from routine tasks in maintaining cell cultures
  • To provide investigators an inexpensive, reliable source of  monoclonal antibodies
  • To provide quality control and surveillance of cell cultures for common tissue culture contaminants (i.e., mycoplasma and endotoxin)
  • To provide expertise for more sophisticated procedures not currently available to all investigators (e.g., construction of viral vectors for gene transfer and immortalization of primary cell cultures with the E6/E7 oncogene)

The tissue culture/hybridoma module is directed by Jerry Niederkorn, Ph.D. Dr. Niederkorn has more than 30 years of experience in tissue culture and hybridoma techniques. He also has experience in developing immortalized primary cell cultures.