Cell Biology & Imaging Core

Cell Biology & Imaging Core sample image

The objectives of the Cell Biology and Imaging Core are to provide high quality histopathologic and scientific support for research projects by localizing proteins and/or mRNAs in tissues and cultured cells, and to offer pathology support. The Core maintains a library of wild-type murine embryos and adult tissues embedded in paraffin for use by O'Brien Center investigators. The lab also maintains bright-field, dark-field, phase-contrast, epifluorescence, and confocal microscopes.

IP-IB Klotho Assay

This assay is provided by the O'Brien Center as a service that incurs non-profit cost-recovery charges. In contrast to the commercial ELISA, we use an immunoprecipitation-immunblot assay using synthetic anti-Klotho antibodies that reliably detects full-length Klotho in sera and urine from human, rat, and mouse.

Compared to the commercial assay, the drawback of our IP-IB assay that it requires higher sample volume and cannot achieve high throughput mode, because of the laborious nature of the assay. However, the main advantage is the highly specific and sensitive reagents and known performance of inter-assay variation of 7 percent and intra-assay variation of < 2 percent.

Cell Biology and Imaging Core Services

Request Services:

  • Klotho IP-IB Assay
  • Imaging of rodent kidney and heart including functional MRI
    • Consultation with Dr. Masaya Takahashi
    • Investigators are responsible for time reserved on the magnet.
      • Apparent diffusion coefficient (fibrosis)
      • Arterial spin labeling (blood flow)
      • BOLD (oxygenation) Na MRS
  • Pathology
    • Processing of tissues for routine paraffin embedding
    • Detailed paraffin and cryo-microtomy
    • Standard histologic stains and special stains when required
    • Immunohistochemistry and in situ hybridization
    • Kidney pathology
    • Single tubule isolation and immunocytochemistry
    • Laser microdissection

Schedule Equipment:

  • Zeiss LSM 510 Meta Confocal Microscope
    • Training provided for the following
      • Image Fixed Cells
      • Image Live Cells
      • Zen Software

For pricing and sample guidelines log in to iLab by clicking Core Request or go to Core Request Instructions to register.

Stained tissue slices