Animal Models Core
The study of genetically modified mice has greatly expanded our understanding of kidney development and pathophysiology. The UT Southwestern O’Brien Center Animal Models Core provides cutting-edge technology to investigators wishing to generate mouse models for the study of kidney development and/or function.
The Animal Models Core serves three major purposes:
- To provide the necessary reagents and expertise to mouse geneticists who do not normally study the kidney, to facilitate and encourage their move into this field.
- To encourage non-mouse geneticists, especially clinical investigators, to venture into the mouse system and to ease this transition.
- To provide tools to mouse geneticists who do study the kidney, to assist in their studies.
The Core generates and distributes several kidney-specific Cre recombinase and tetracycline-inducible activator and repressor lines to be crossed to co-investigator-provided floxed or tet responder lines. Core personnel assist in the generation of additional transgenic mice, knockout/in mice, and cell lines, as required by co-investigators. Core personnel also use and train investigators to use various models of organ injury/repair and ex vivo culture. As mentioned, the ultimate purpose of this core is to facilitate research on kidney development and pathophysiology.
- Distribution of transgenic mice expressing kidney-specific Cre recombinase, including:
- Ksp/CreERT2 (available through Jackson Laboratories)
- Generation and distribution of transgenic mice expressing kidney-specific tetracycline-inducible activator and repressor:
- Distribution of Kif3a mutant and wildtype cell lines
- Assistance in the generation of transgenic mice and knock-out/knock-in mice
- Performing and training investigators in the use of kidney injury/repair models, including renal ischemia-reperfusion injury, ureteral obstruction, and chemical injury
- Training investigators to perform embryonic kidney organ cultures